Isolation of subtelomeric DNA sequences labelling sheep and goat chromosome ends

Genetics Selection Evolution

Edmond P Cribiu³  Philippe Metezeau²  Allan Crawford¹  Anne Vaiman³  Céline Derbois³  Mohamed Bensaada²  Ana Brunialti³  Daniel Vaiman³ 

[1] AgResearch Molecular Biology Unit, University of Otago, P.O. Box 56 Dunedin, New Zealand;Laboratoire de cytométrie analytique, Institut Pasteur, 25 rue du Dr Roux, 75014 Paris, France;Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex, France

关键词: goats;    sheep;    FISH;    telomeres;   

Others  :  1094733
DOI  :  10.1186/1297-9686-32-6-599

 received in 2000-05-03, accepted in 2000-09-15,  发布年份 2000

【 摘 要 】

Two techniques that make it possible to isolate telomere DNA are presented, using sheep as an example. The first technique is based upon the screening of a sheep BAC library with PCR amplified DNA segments preserved from high-power laser beam irradiation. Twenty-three BACs hybridising to 13 subtelomeric regions in sheep and goats were obtained (out of 27 in the sheep complement), of which 13 recognised more than one region, telomeric or not. Twenty-three microsatellites were isolated from these BACs and 22 were genetically mapped on the sheep international genetic map, always consistently with the cytogenetical localisation in 17 cases out of 22. These results are discussed. The second technique is based upon the selective cloning of subtelomeric enriched DNA. Preliminary results were obtained by this approach.

【 授权许可】

   
2000 INRA, EDP Sciences