Protective and antidiabetic effects of extract from Nigella sativa on blood glucose concentrations against streptozotocin (STZ)-induced diabetic in rats: an experimental study with histopathological evaluation

Diagnostic Pathology

Hamid Akbari⁸  Farshid Khadivar²  Maryam Tavakoli³  Reza Mortezaee¹  Danial Kheradmand⁷  Javad Javanbakht⁴  Rahim Hobbenaghi⁵  Samad Alimohammadi⁶ 

[1] Young Researchers Club and Elites, Mashhad Branch, Islamic Azad University, Mashahd, Iran;Faculty of Veterinary Medicine, Tehran University, Tehran, Iran;Graduate Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;Department of Pathology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran;Department of Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;Department of Physiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran;Faculty of Medicine MD, Graduate Student of Islamic Azad University of Mashhad, Mashhad, Iran;Department of Clinical Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

关键词: Rat;    Hypoglycemic;    Streptozotocin;    Nigella Sativa;   

Others  :  805566
DOI  :  10.1186/1746-1596-8-137

 received in 2013-07-29, accepted in 2013-08-08,  发布年份 2013

【 摘 要 】

Background

Diabetes in humans induces chronic complications such as cardiovascular damage, cataracts and retinopathy, nephropathy and polyneuropathy. The most common animal model of human diabetes is streptozotocin (STZ)-induced diabetes in the rat. The present study investigated the effects of Nigella sativa hydroalcholic extract on glucose concentrations in streptozotocin (STZ) diabetic rats.

Methods

In this study Twenty-five Wister-Albino rats (aged 8-9 weeks and weighing 200-250 g) were tested. Rats were divided into five experimental groups (control, untreated STZ-diabetic (60 mg/kg B.W., IP), treated STZ-diabetic with hydroalcholic extract of Nigella Sativa (NS) (5 mg/kg B.W, IP), treated STZ-diabetic with hydroalcholic extract of NS (10 mg/kg B.W., IP) and treated STZ-diabetic with hydroalcholic extract of NS (20 mg/kg B.W., IP and 32 days were evaluated to assess its effect on fasting blood glucose (FBG), and in different groups fasting blood glucose (FBG) and body weight (BW) were measured in the particular days (1, 16 and 32). At the end of the study, the animals were fasted overnight, anaesthetized with an intraperitoneal injection of sodium pentobarbital (60 mg/kg), and sacrificed for obtaining tissues samples (liver, pancreases). The number of islets and cells were counted and the islet diameters were determined by calibrated micrometer. The glycogen content in the liver was examined by Periodic Acid-Schiff (PAS) staining.

Results

Treatment with NS (5 mg/kg b.w.) markedly increased BW gain and the FBG level was significantly (p<0.001) reduced when compared to the control. Histopathological examination showed that the NS (5 mg/kg b.w.) partially recovered hepatic glycogen content and protected the great deal of the pancreatic islet cells. The number of islets, cells and islets diameter were found statistically significant when compared to the control (p<0.01, p<0.05).

Conclusions

Higher doses of NS did not exhibit any therapeutic effect. These results showed that hydroalcholic extract of NS at low doses has hypoglycemic effect and ameliorative effect on regeneration of pancreatic islets and may be used as a therapeutic agent in the management of diabetes mellitus. The hypoglycemic effect observed could be due to amelioration of β-cell, thus leading to increased insulin levels. Consequently, N. sativa may prove clinically useful in the treatment of diabetics and in the protection of β-cells against streptozotocin.

Virtual slide

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1845133011104231 webcite

【 授权许可】

   
2013 Alimohammadi et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Swamy SMK, Tan BKH: Cytotoxic and immunopotentiating effects of ethanolic extract of Nigella sativa L. seeds. J Ethnopharmacol 2000, 70:1-7.
• [2]Boulos L: Medicinal plants of North Africa. Algonac, MI: Reference Publications; 1983:103.
• [3]Houghton PJ, Zakara R, Heras B, Hoult JR: Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 1995, 61:33-36.
• [4]Agarwal R, Kharya MD, Shrivastava R: Antimicrobial and anti-helminthic activities of the essential oil of Nigella sativa Linn. Ind J Exp Biol 1979, 17:1264-1265.
• [5]Al-Hader A, Aqel M, Hasan Z: Hypoglycemic effect of the volatile oil of Nigella sativa seeds. Int J Pharmacog 1993, 31:96-100.
• [6]Haq A, Lobo PI, Al-Tufail M, Rama N, Al-Sedairy S: Immunomodulatory effect of Nigella sativa proteins fractionated by ion exchange chromatography. Int J Immunopharmacol 1999, 21:283-295.
• [7]Javanbakht J, Hobbenaghi R, Hosseini E, Bahrami AM, Khadivar F, Fathi S, Hassan MA: Histopathological investigation of neuroprotective effects of Nigella sativa on motor neurons anterior horn spinal cord after sciatic nerve crush in rats. Pathol Biol (Paris) 2013.
• [8]Van Bremen T, Drömann D, Luitjens K, Dodt C, Dalhoff K, Goldmann T, Schaaf B: Triggering receptor expressed on myeloid cells-1 (Trem-1) on blood neutrophils is associated with cytokine inducibility in human E. coli sepsis. Diagn Pathol 2013, 8:24. BioMed Central Full Text
• [9]Ramakrishna V, Jailkhani R: Evaluation of oxidative stress in Insulin Dependent Diabetes Mellitus (IDDM) patients. Diagn Pathol 2007, 2:22. BioMed Central Full Text
• [10]Otani N, Akimoto T, Yumura W, Matsubara D, Iwazu Y, Numata A, Miki T, Takemoto F, Fukushima N, Muto S, Kusano E: Is there a link between diabetic glomerular injury and crescent formation? A case report and literature review. Diagn Pathol 2012, 7:46. BioMed Central Full Text
• [11]Baurakiades E, Martins AP, Victor Moreschi N, Souza CD, Abujamra K, Saito AO, Mecatti MC, Santos MG, Pimentel CR, Silva LL, Cruz CR, de Noronha L: Histomorphometric and immunohistochemical analysis of infectious agents, T-cell subpopulations and inflammatory adhesion molecules in placentas from HIV-seropositive pregnant women. Diagn Pathol 2011, 6:101. BioMed Central Full Text
• [12]Baynes JW, Thorpe SR: The role of oxidative stress in diabetic complications. Curr Opin Endocrinol 1996, 3:277-84.
• [13]Baynes JW: Role of oxidative stress in development of complications in diabetes. Diabetes 1991, 40:405-12.
• [14]Gillery P, Monboisse JC, Maquart FX, Borel JP: Does oxygen free radical increased formation explain longterm complications of Diabetes mellitus? Med Hypotheses 1989, 29:47-50.
• [15]Seven A, Guzel S, Seymen O, Civelek S, Bolayirli M, Yigit G: Nitric oxide synthase inhibition by L-NAME in streptozotocin induced diabetic rats: impacts on oxidative stress. Tohoku J Exp Med 2003, 99:205-10.
• [16]Matkovics B, Kotorman M, Varga IS, Hai DQ, Varga C: xidative stress in experimental diabetes induced by streptozotocin. Acta Physiol Hung 1997–1998, 85:29-38.
• [17]Kanter M, Coskun O, Korkmaz A: Effects of Nigella sativa on oxidative stress and β-cell damage in streptozotocin-induced diabetic rats. Anat Rec A Discov Mol Cell Evol Biol 2004, 279A(1):685-691.
• [18]Fararh KM, Atoji Y, Shimizu Y: Isulinotropic properties of Nigella sativa oil in streptozotocin plus nicotinamide diabetic hamster. Research Veterinary Science 2002, 73:279-282.
• [19]Alsaif MA: Effect of Nigella sativa oil on impaired glucose tolerance and insulin insensitivity induced by high-fat-diet and turpentine-induced trauma. Pak J Biol Sci 2008, 11(8):1093-9.
• [20]El-Dakhakhny M, Mady N, Lembert N, Ammon HP: The hypoglycemic effect of Nigella sativa oil is mediated by extrapancreatic actions. Plant Med 2002, 68(5):465-6.
• [21]Abdelmeguid NE, Fakhoury R, Kamal SM, Al Wafai RJ: Effects of Nigella sativa and thymoquinone on biochemical and subcellular changes in pancreatic β-cells of streptozotocin-induced diabetic rats. Diabetes 2010, 2(4):256-66.
• [22]Geng D, Zhang S, Lan J: Analysis on chemical components of volatile oil and determination of thymoquinone from seed of Nigella glandulifera. Zhongguo Zhong Yao Za Zhi 2009, 34(22):2887-90.
• [23]Pari L, Sankaranarayanan C: Beneficial effects of thymoquinone on hepatic key enzymes in streptozotocin–nicotinamide induced diabetic rats. Life Sci 2009, 85(23–26):830-834.
• [24]Grover J, Vats V, Yadav S: Effect of feeding aqueous extract of Pterocarpus marsupium on glycogen content of tissues and the key enzymes of carbohydrate metabolism. Mol Cell Biochem 2002, 241:53-59.