Risk factors associated with negative in-vivo diagnostic results in bovine tuberculosis-infected cattle in Spain

BMC Veterinary Research

Lucas Domínguez⁴  Lucía de Juan⁴  Olga Mínguez¹  Maria del Carmen Martínez¹  Maria del Rosario Esquivel¹  Jose Luis Saez⁵  Beatriz Romero²  Maria Luisa de la Cruz²  Anna Grau¹  Javier Bezos²  Sergio Marqués¹  Andrés Perez³  Julio Álvarez² 

[1] Dirección General de Producción Agropecuaria, Servicio de Sanidad Animal, Junta de Castilla y León, C/ Rigoberto Cortejoso 14, 47014 Valladolid, Spain;Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense Madrid, 28040 Madrid, Spain;CONICET/Facultad de Ciencias Veterinarias UNR, Casilda, Santa Fe, Argentina;Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain;Subdirección General de Sanidad e Higiene Animal y Trazabilidad, Ministerio de Agricultura, Alimentación y Medio Ambiente, 28071 Madrid, Spain

关键词: Risk factors;    Interferon-gamma assay;    Single tuberculin test;    Diagnosis;    Cattle;    Tuberculosis;   

Others  :  1119356
DOI  :  10.1186/1746-6148-10-14

 received in 2013-01-31, accepted in 2014-01-03,  发布年份 2014

【 摘 要 】

Background

Despite great effort and investment incurred over decades to control bovine tuberculosis (bTB), it is still one of the most important zoonotic diseases in many areas of the world. Test-and-slaughter strategies, the basis of most bTB eradication programs carried out worldwide, have demonstrated its usefulness in the control of the disease. However, in certain countries, eradication has not been achieved due in part to limitations of currently available diagnostic tests. In this study, results of in-vivo and post-mortem diagnostic tests performed on 3,614 animals from 152 bTB-infected cattle herds (beef, dairy, and bullfighting) detected in 2007–2010 in the region of Castilla y León, Spain, were analyzed to identify factors associated with positive bacteriological results in cattle that were non-reactors to the single intradermal tuberculin test, to the interferon-gamma (IFN-γ) assay, or to both tests applied in parallel (Test negative/Culture + animals, T-/C+). The association of individual factors (age, productive type, and number of herd-tests performed since the disclosure of the outbreak) with the bacteriology outcome (positive/negative) was analyzed using a mixed multivariate logistic regression model.

Results

The proportion of non-reactors with a positive post-mortem result ranged from 24.3% in the case of the SIT test to 12.9% (IFN-γ with 0.05 threshold) and 11.9% (95% CI 9.9-11.4%) using both tests in parallel. Older (>4.5 years) and bullfighting cattle were associated with increased odds of confirmed bTB infection by bacteriology, whereas dairy cattle showed a significantly lower risk. Ancillary use of IFN-γ assay reduced the proportion of T-/C + animals in high risk groups.

Conclusions

These results demonstrate the likelihood of positive bacteriological results in non-reactor cattle is influenced by individual epidemiological factors of tested animals. Increased surveillance on non-reactors with an increased probability of being false negative could be helpful to avoid bTB persistence, particularly in chronically infected herds. These findings may aid in the development of effective strategies for eradication of bTB in Spain.

【 授权许可】

   
2014 Álvarez et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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