Genome scan study of prostate cancer in Arabs: identification of three genomic regions with multiple prostate cancer susceptibility loci in Tunisians

Journal of Translational Medicine

Lotfi Chouchane¹  Idil I Aigha¹  Shahzad Jafri⁴  Hanif G Khalak⁴  Jillian Rowe⁴  Shoba P Dsouza¹  Hala Kfoury³  Sami Al-Said⁵  Karim Farhat³  Dhanya Kizhakayil¹  Issam Al-Bozom²  Danny Rabah³  Khalid Al-Rumaihi⁵  Jingxuan Shan¹ 

[1] Laboratory of Genetic Medicine and Immunology, Weill Cornell Medical College in Qatar, Qatar Foundation, Doha, Qatar;Department of Laboratory Medicine and Pathology, Hamad Medical Corporation, Doha, Qatar;Princess Al-Jouhara Al-Ibrahim Cancer Research Center, College of Medicine and King, Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia;Information Technology Services Division, Weill Cornell Medical College in Qatar, Qatar Foundation, Doha, Qatar;Department of Surgery, Hamad Medical Corporation, Doha, Qatar

关键词: Arab population;    GWAS;    Prostate cancer;   

Others  :  827554
DOI  :  10.1186/1479-5876-11-121

 received in 2013-02-14, accepted in 2013-05-02,  发布年份 2013

【 摘 要 】

Background

Large databases focused on genetic susceptibility to prostate cancer have been accumulated from population studies of different ancestries, including Europeans and African-Americans. Arab populations, however, have been only rarely studied.

Methods

Using Affymetrix Genome-Wide Human SNP Array 6, we conducted a genome-wide association study (GWAS) in which 534,781 single nucleotide polymorphisms (SNPs) were genotyped in 221 Tunisians (90 prostate cancer patients and 131 age-matched healthy controls). TaqMan^® SNP Genotyping Assays on 11 prostate cancer associated SNPs were performed in a distinct cohort of 337 individuals from Arab ancestry living in Qatar and Saudi Arabia (155 prostate cancer patients and 182 age-matched controls). In-silico expression quantitative trait locus (eQTL) analysis along with mRNA quantification of nearby genes was performed to identify loci potentially cis-regulated by the identified SNPs.

Results

Three chromosomal regions, encompassing 14 SNPs, are significantly associated with prostate cancer risk in the Tunisian population (P = 1 × 10^-4 to P = 1 × 10^-5). In addition to SNPs located on chromosome 17q21, previously found associated with prostate cancer in Western populations, two novel chromosomal regions are revealed on chromosome 9p24 and 22q13. eQTL analysis and mRNA quantification indicate that the prostate cancer associated SNPs of chromosome 17 could enhance the expression of STAT5B gene.

Conclusion

Our findings, identifying novel GWAS prostate cancer susceptibility loci, indicate that prostate cancer genetic risk factors could be ethnic specific.

【 授权许可】

   
2013 Shan et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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