Rabbiteye blueberry prevents osteoporosis in ovariectomized rats

Journal of Orthopaedic Surgery and Research

Sheng Ou-Yang¹  Zhi-Yuan Xu¹  Shou-Mian Wu¹  Tao Li² 

[1] Department of Emergency, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China;Department of Orthopedics, The Third Affiliated Hospital of Southern Medical University (Academy of Orthopedics), Guangzhou 510630, Guangdong Province, China

关键词: Bone histomorphometry;    Focal adhesion kinase;    Alkaline phosphatase activity;    Osteoporosis;    Rabbiteye blueberry;   

Others  :  1152336
DOI  :  10.1186/s13018-014-0056-9

 received in 2014-01-20, accepted in 2014-06-20,  发布年份 2014

【 摘 要 】

Objective

It has been forecasted that the rabbiteye blueberry could inhibit osteoporosis. However, the inhibition and prevention of osteoporosis via rabbiteye blueberry are still elusive. This study was aim to evaluate the anti-osteoporosis effects of rabbiteye blueberry in ovariectomized rats.

Methods

Thirty rats were randomly divided into three groups of ten rats each as follows: sham-operated group (SG), ovariectomized model control group (OMG), and ovariectomized rabbiteye blueberry treatment group (OBG). The blood mineral levels, the alkaline phosphatase (ALP) activity, and osteoprotegerin (OPG) level were determined. The expression analyses of type I collagen, integrin-β1, and focal adhesion kinase (FAK) were performed. Besides, the bone mineral density (BMD) and bone histomorphometry (BH) were measured.

Results

The ALP activity in SG and OBG was significantly lower than that in OMG. For the OPG level, the significant increase of OPG level in OBG was indicated compared with the other groups. The mRNA expression levels of type I collagen, integrin-β1, and FAK in OMG were significantly lower than those in other groups. The BMD in OMG were all significantly lower than those in SG and OBG. For BH, blueberry significantly improved the trabecular bone volume fraction, trabecular thickness, mean trabecular bone number, and bone formation rate, and decreased the trabecular separation, the percent of bone resorption perimeter, and mean osteoclast number in OBG compared with OMG.

Conclusions

The rabbiteye blueberries had an effective inhibition in bone resorption, bone loss, and reduction of bone strength of ovariectomized rats and could improve the BMD, osteogenic activity, and trabecular bone structure.

【 授权许可】

   
2014 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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【 参考文献 】

• [1]Prevention and management of osteoporosis: report of a WHO Scientific Group World Health Organ Tech Rep Ser 2003, 921:1-164.
• [2]Brömme D, Lecaille F: Cathepsin K inhibitors for osteoporosis and potential off-target effects. Expert Opin Investig Drugs 2009, 18:585-600.
• [3]Tafaro L, Nati G, Leoni E, Baldini R, Cattaruzza M, Mei M, Falaschi P: Adherence to anti-osteoporotic therapies: role and determinants of “spot therapy”. Osteoporos Int 2013, 24(8):2319-2323.
• [4]Taylor K, Middlefell L, Mizen K: Osteonecrosis of the jaws induced by anti-RANK ligand therapy. Br J Oral Maxillofac Surg 2010, 48(3):221-223.
• [5]Boonen S, Rosenberg E, Claessens F, Vanderschueren D, Papapoulos S: Inhibition of cathepsin K for treatment of osteoporosis. Curr Osteoporos Rep 2012, 10(1):73-79.
• [6]McDermott MT: Osteoporosis 2011: Compromised Bone Strength Predisposing to Increased Risk of Fragility and Fractures. University of Colorado Hospital, Aurora; 2011.
• [7]Armitage JM, Bowman L, Clarke RJ, Wallendszus K, Bulbulia R, Rahimi K, Haynes R, Parish S, Sleight P, Peto R: Effects of homocysteine-lowering with folic acid plus vitamin B12 vs placebo on mortality and major morbidity in myocardial infarction survivors. JAMA 2010, 303(24):2486-2494.
• [8]Baines M, Kredan M, Usher J, Davison A, Higgins G, Taylor W, West C, Fraser W, Ranganath L: The association of homocysteine and its determinants MTHFR genotype, folate, vitamin B12 and vitamin B6 with bone mineral density in postmenopausal British women. Bone 2007, 40(3):730-736.
• [9]Sugiura M, Nakamura M, Ogawa K, Ikoma Y, Yano M: High serum carotenoids associated with lower risk for bone loss and osteoporosis in post-menopausal Japanese female subjects: prospective cohort study. PLoS One 2012, 7(12):e52643.
• [10]Sahni S, Hannan MT, Blumberg J, Cupples LA, Kiel DP, Tucker KL: Protective effect of total carotenoid and lycopene intake on the risk of hip fracture: a 17‐year follow‐up from the Framingham Osteoporosis Study. J Bone Miner Res 2009, 24(6):1086-1094.
• [11]Sugiura M, Nakamura M, Ogawa K, Ikoma Y, Ando F, Shimokata H, Yano M: Dietary patterns of antioxidant vitamin and carotenoid intake associated with bone mineral density: findings from post-menopausal Japanese female subjects. Osteoporos Int 2011, 22(1):143-152.
• [12]Zhang J, Lazarenko OP, Blackburn ML, Shankar K, Badger TM, Ronis MJ, Chen J-R: Feeding blueberry diets in early life prevent senescence of osteoblasts and bone loss in ovariectomized adult female rats. PLoS One 2011, 6(9):e24486.
• [13]Cooper C: Osteoporosis: disease severity and consequent fracture management. Osteoporos Int 2010, 21(2):425-429.
• [14]Osako MK, Nakagami H, Koibuchi N, Shimizu H, Nakagami F, Koriyama H, Shimamura M, Miyake T, Rakugi H, Morishita R: Estrogen inhibits vascular calcification via vascular RANKL system: common mechanism of osteoporosis and vascular calcification. Circ Res 2010, 107(4):466.
• [15]Manolagas SC: From estrogen-centric to aging and oxidative stress: a revised perspective of the pathogenesis of osteoporosis. Endocr Rev 2010, 31(3):266-300.
• [16]Kanis J, Black D, Cooper C, Dargent P, Dawson-Hughes B, De Laet C, Delmas P, Eisman J, Johnell O, Jonsson B: A new approach to the development of assessment guidelines for osteoporosis. Osteoporos Int 2002, 13(7):527-536.
• [17]Ramirez MR, Guterres L, Dickel OE, de Castro MR, Henriques AT, de Souza MM, Barros DM: Preliminary studies on the antinociceptive activity of Vaccinium ashei berry in experimental animal models. J Med Food 2010, 13(2):336-342.
• [18]Inoue N, Nagao K, Nomura S, Shirouchi B, Inafuku M, Hirabaru H, Nakahara N, Nishizono S, Tanaka T, Yanagita T: Effect of Vaccinium ashei Reade leaf extracts on lipid metabolism in obese OLETF rats. Biosci Biotechnol Biochem 2011, 75(12):2304-2308.
• [19]Shen C-L, Yeh J, Cao J, Tatum O, Dagda R, Wang J-S: Synergistic effects of green tea polyphenols and alphacalcidol on chronic inflammation-induced bone loss in female rats. Osteoporos Int 2010, 21(11):1841-1852.
• [20]Shuid AN, Ping LL, Muhammad N, Mohamed N, Soelaiman IN: The effects of Labisia pumila var. alata on bone markers and bone calcium in a rat model of post-menopausal osteoporosis. J Ethnopharmacol 2011, 133(2):538-542.
• [21]Gennari L, Merlotti D, Martini G, Gonnelli S, Franci B, Campagna S, Lucani B, Dal Canto N, Valenti R, Gennari C, Nuti R: Longitudinal association between sex hormone levels, bone loss, and bone turnover in elderly men. J Clin Endocrinol Metab 2003, 88(11):5327-5333.
• [22]Otto F, Lubbert M, Stock M: Upstream and downstream targets of RUNX proteins. J Cell Biochem 2003, 89(1):9-18.
• [23]Bucay N, Sarosi I, Dunstan CR, Morony S, Tarpley J, Capparelli C, Scully S, Tan HL, Xu W, Lacey DL, Boyle WJ, Simonet WS: Osteoprotegerin-deficient mice develop early onset osteoporosis and arterial calcification. Genes Dev 1998, 12(9):1260-1268.
• [24]Shekaran A, García AJ: Extracellular matrix‐mimetic adhesive biomaterials for bone repair. J Biomed Mater Res A 2011, 96(1):261-272.
• [25]Mizuno M, Fujisawa R, Kuboki Y: Type I collagen‐induced osteoblastic differentiation of bone‐marrow cells mediated by collagen‐α2β1 integrin interaction. J Cell Physiol 2000, 184(2):207-213.
• [26]Reyes CD, García AJ: α2β1 integrin-specific collagen-mimetic surfaces supporting osteoblastic differentiation. J Biomed Mater Res A 2004, 69(4):591-600.
• [27]Garnero P, Gineyts E, Riou JP, Delmas PD: Assessment of bone resorption with a new marker of collagen degradation in patients with metabolic bone disease. J Clin Endocrinol Metab 1994, 79(3):780-785.
• [28]Risteli J, Niemi S, Elomaa I, Risteli L: Bone resorption assay based on a peptide liberated during type I collagen degradation. J Bone Miner Res 1991, 6(Suppl 1):S251.
• [29]Riggs BL, Khosla S, Melton LJ III: Sex steroids and the construction and conservation of the adult skeleton. Endocr Rev 2002, 23(3):279-302.
• [30]Francis DA, Millis DL, Head LL: Bone and lean tissue changes following cranial cruciate ligament transection and stifle stabilization. J Am Anim Hosp Assoc 2006, 42(2):127-135.
• [31]David V, Laroche N, Boudignon B, Lafage‐Proust MH, Alexandre C, Ruegsegger P, Vico L: Noninvasive in vivo monitoring of bone architecture alterations in hindlimb‐unloaded female rats using novel three‐dimensional microcomputed tomography. J Bone Miner Res 2003, 18(9):1622-1631.
• [32]Ding M, Hvid I: Quantification of age-related changes in the structure model type and trabecular thickness of human tibial cancellous bone. Bone 2000, 26(3):291-295.
• [33]Burguera B, Hofbauer LC, Thomas T, Gori F, Evans GL, Khosla S, Riggs BL, Turner RT: Leptin reduces ovariectomy-induced bone loss in rats. Endocrinology 2001, 142(8):3546-3553.
• [34]Jilka RL, Hangoc G, Girasole G, Passeri G, Williams DC, Abrams JS, Boyce B, Broxmeyer H, Manolagas SC: Increased osteoclast development after estrogen loss: mediation by interleukin-6. Science 1992, 257(5066):88-91.