Effects of some anesthetic agents on skin microcirculation evaluated by laser Doppler perfusion imaging in mice

BMC Veterinary Research

Giancarlo Vesce³  Arturo Brunetti¹  Adelaide Greco¹  Raffaele Liuzzi²  Matteo Gramanzini¹  Sara Gargiulo¹ 

[1] CEINGE scarl, Via G. Salvatore 486, Naples 80145, Italy;Department of Advanced Biomedical Sciences, University of Naples Federico II, Via Pansini 5, Naples 80145, Italy;Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Delpino 1, Naples 80137, Italy

关键词: Laser Doppler perfusion imaging;    Murine model;    Anesthesia;    Microvascular perfusion;   

Others  :  1119383
DOI  :  10.1186/1746-6148-9-255

 received in 2013-09-09, accepted in 2013-12-02,  发布年份 2013

【 摘 要 】

Background

Anesthetic agents alter microcirculation, influencing tissue oxygenation and delivery of vital substrates. Laser Doppler perfusion imaging is a widespread technique in the field of microvascular research that can evaluate noninvasively and in real time the effects of environmental conditions, physical manipulations, diseases and treatments on peripheral perfusion. This study aims to evaluate laser Doppler perfusion imaging as a means to detect changes in skin microcirculation induced by some popular anesthetic agents in a murine model. Twenty-four age- and gender-matched healthy CD1 mice were examined by laser Doppler perfusion imaging. The skin microcirculatory response was measured at the level of plantar surfaces during isoflurane anesthesia with or without subsequent dexmedetomidine or acepromazine. At the end of the procedure, dexmedetomidine was reversed by atipamezole administration.

Results

In all mice, skin blood flow under isoflurane anesthesia did not show significant differences over time (P = 0.1). The serial perfusion pattern and values following acepromazine or dexmedetomidine administration differed significantly (P < 0.05).

Conclusions

We standardized a reliable laser Doppler perfusion imaging protocol to non-invasively assess changes in skin microcirculation induced by anesthesia in mice, considering the advantages and drawbacks of this technique and its translational value.

【 授权许可】

   
2013 Gargiulo et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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