Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers

Journal of Animal Science and Biotechnology

Jianhui Tian¹  Lei An¹  Zhonghong Wu¹  Xiaoling Xu¹  Haichao Zhao¹  Yunwei Pang¹  Yong Yu¹ 

[1] Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, PR China

关键词: Superovulation;    SNP;    LHCGR;    Chinese Holstein heifer;   

Others  :  813363
DOI  :  10.1186/2049-1891-3-35

 received in 2012-04-06, accepted in 2012-08-07,  发布年份 2012

【 摘 要 】

Background

Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation.

Methods

Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers.

Results

A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos.

Conclusions

LHCGR may be a new predictor for superovulation in Chinese Holstein heifers.

【 授权许可】

   
2012 Yu et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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