One nanoprobe, two pathogens: gold nanoprobes multiplexing for point-of-care

Journal of Nanobiotechnology

Pedro V Baptista²  Maria M Mota¹  Elvira Fortunato³  Ana Rita Grosso¹  Liliana Mancio-Silva¹  Fábio F Carlos⁴  Pedro Pedrosa²  Bruno Veigas³ 

[1] Instituto de Medicina Molecular, Universidade de Lisboa. Av. Prof. Egas Moniz, Lisbon, 1649-028, Portugal;Nanomedicine@FCT, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, CIGMH, UCIBIO, Universidade Nova de Lisboa, Campus de Caparica, Caparica, 2829-516, Portugal;Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, CENIMAT/I3N, Universidade Nova de Lisboa, Caparica, Portugal;STABVIDA, Investigação e Serviços em Ciências Biológicas, Lda. Madan Parque, Caparica, 2825-182, Portugal

关键词: Plasmodium;    rpoB;    Tuberculosis;    Gold nanoparticles;    Nanodiagnostics;    Malaria;    MTBC;   

Others  :  1224853
DOI  :  10.1186/s12951-015-0109-1

 received in 2015-03-28, accepted in 2015-07-09,  发布年份 2015

【 摘 要 】

Background

Gold nanoparticles have been widely employed for biosensing purposes with remarkable efficacy for DNA detection. Amongst the proposed systems, colorimetric strategies based on the remarkable optical properties have provided for simple yet effective sequence discrimination with potential for molecular diagnostics at point of need. These systems may also been used for parallel detection of several targets to provide additional information on diagnostics of pathogens.

Results

For the first time, we demonstrate that a single Au-nanoprobe may provide for detection of two distinct targets (pathogens) allowing colorimetric multi-target detection. We demonstrate this concept by using one single gold-nanoprobe capable to detect members of the Mycobacterium tuberculosis complex and Plasmodium sp., the etiologic agents of tuberculosis and malaria, respectively. Following characterisation, the developed gold-nanoprobe allowed detection of either target in individual samples or in samples containing both DNA species with the same efficacy.

Conclusions

Using one single probe via the non-cross-linking colorimetric methodology it is possible to identify multiple targets in one sample in one reaction. This proof-of-concept approach may easily be integrated into sensing platforms allowing for fast and simple multiplexing of Au-nanoprobe based detection at point-of-need.

【 授权许可】

   
2015 Veigas et al.

【 图 表 】

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