Elevated expression of chloride intracellular channel 1 is correlated with poor prognosis in human gliomas

Journal of Experimental & Clinical Cancer Research

Guodong Gao¹  Yongsheng Zhang²  Jipei Zhao¹  Fuqiang Feng¹  Jingyu Zhang¹  Jianhai Zong¹  Peigang Ji¹  Yanyang TU²  Shiming He¹  Liang Wang¹ 

[1] Department of neurosurgery, Tangdu hospital, Fourth Military Medical University of PLA, No.569, Xinsi Road, Baqiao District, Xi’an City, 710038, China;Department of experimental surgery, Tangdu hospital, Fourth Military Medical University of PLA, No.569, Xinsi Road, Baqiao District, Xi’an City, 710038, China

关键词: Prognosis;    Immunohistochemistry;    Real-time quantitative RT-PCR assay;    Glioma;    Chloride intracellular channel 1;   

Others  :  826441
DOI  :  10.1186/1756-9966-31-44

 received in 2012-03-28, accepted in 2012-05-05,  发布年份 2012

【 摘 要 】

Background

Chloride intracellular channel 1 (CLIC1) is expressed ubiquitously in human tissues and is involved in the regulation of cell cycle, cell proliferation and differentiation. Recent studies have shown that CLIC1 is highly expressed in several human malignant tumors. However, its roles in human gliomas are still unclear. The aim of this study was to investigate the clinicopathological significance and prognostic value of CLIC1 expression in human gliomas.

Methods

CLIC1 expression in human gliomas and nonneoplastic brain tissues was measured by real-time quantitative RT-PCR assay and immunohistochemistry. Its association with clinicopathological factors or prognosis in patients with gliomas was statistically analyzed.

Results

The expression of CLIC1 at both mRNA and protein levels was significantly increased in high-grade (Grade III~IV) glioma tissues compared with that in low-grade (Grade I~II) and nonneoplastic brain tissues, and was up-regulated with ascending tumor World Health Organization (WHO) grades. The elevated expression of CLIC1 protein was also significantly correlated with low Karnofsky performance score (KPS) (P=0.008). Moreover, both univariate and multivariate analysis shown that high CLIC1 expression was significantly associated with poor prognosis in patients with gliomas (P<0.001 and P=0.01, respectively). In particular, the elevated CLIC1 expression also correlated with shorter overall survival in different glioma subgroups stratified according to the WHO grading.

Conclusions

Our data provide the first evidence that CLIC1 expression might play an important role in the regulation of aggressiveness in human gliomas. The elevated expression of CLIC1 might represent a valuable prognostic marker for this disease.

【 授权许可】

   
2012 Wang et al.; licensee BioMed Central Ltd.

【 图 表 】

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