Biodiversity of 52 chicken populations assessed by microsatellite typing of DNA pools

Genetics Selection Evolution

Steffen Weigend²  Klaus Wimmers¹  Alain Vignal⁷  Pippa Thomson³  Marian Oortwijn⁴  Asko Mäki-Tanila⁶  Paul J Freidlin⁵  Marcus W Feldman⁹  Kari Elo⁶  Richard PMA Crooijmans⁴  Asili Barre-Dirie²  Terry Burke³  Valery M Kirzhner^1,10  Lior David⁵  Abraham B Korol^1,10  Michèle Tixier-Boichard⁸  Martien AM Groenen⁴  Jossi Hillel⁵ 

[1] Institute of Animal Breeding Science, Rheinische Friedrich-Wilhelms-Universitat Bonn, Germany;Institute for Animal Science, Federal Agricultural Research Centre, Mariensee, 31535 Neustadt, Germany;Department of Animal and Plant Sciences, Sheffield University, S10 2TN, UK;Institute of Animal Sciences, Wageningen Agricultural University, Wageningen, The Netherlands;Department of Genetics, The Hebrew University of Jerusalem, Faculty of Agriculture, Food and Environmental quality sciences, Rehovot 76100, Israel;Agricultural Research Centre, Institute of Animal Production, 31600 Jokioinen, Finland;Institut national de la recherche agronomique, Centre de Toulouse, France;Institut national de la recherche agronomique, Centre de Jouy-en-Josas, France;Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA;Institute of Evolution, University of Haifa, Israel

关键词: domesticated chicken;    DNA markers;    red jungle fowl;    polymorphism;    genetic distance;   

Others  :  1094406
DOI  :  10.1186/1297-9686-35-6-533

 received in 2002-09-02, accepted in 2003-03-13,  发布年份 2003

【 摘 要 】

In a project on the biodiversity of chickens funded by the European Commission (EC), eight laboratories collaborated to assess the genetic variation within and between 52 populations from a wide range of chicken types. Twenty-two di-nucleotide microsatellite markers were used to genotype DNA pools of 50 birds from each population. The polymorphism measures for the average, the least polymorphic population (inbred C line) and the most polymorphic population (Gallus gallus spadiceus) were, respectively, as follows: number of alleles per locus, per population: 3.5, 1.3 and 5.2; average gene diversity across markers: 0.47, 0.05 and 0.64; and proportion of polymorphic markers: 0.91, 0.25 and 1.0. These were in good agreement with the breeding history of the populations. For instance, unselected populations were found to be more polymorphic than selected breeds such as layers. Thus DNA pools are effective in the preliminary assessment of genetic variation of populations and markers. Mean genetic distance indicates the extent to which a given population shares its genetic diversity with that of the whole tested gene pool and is a useful criterion for conservation of diversity. The distribution of population-specific (private) alleles and the amount of genetic variation shared among populations supports the hypothesis that the red jungle fowl is the main progenitor of the domesticated chicken.

【 授权许可】

   
2003 INRA, EDP Sciences