Biocompatibility and in vivo osteogenic capability of novel bone tissue engineering scaffold A-W-MGC/CS

Journal of Orthopaedic Surgery and Research

Dan-Ping Liu²  Zheng Zhang²  Guo-Xian Wang¹  Chen Li³ 

[1] Department of Pharmacology, Liaoning Medical University, Jinzhou 121000, China;Department of Orthopaedic Surgery, First Affiliated Hospital of Liaoning Medical University, No. 2 Wuduan Renmin Street Guta District, Jinzhou 121001, China;Biobank, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China

关键词: Repair;    Bone defect;    A-W-MGC/CS;    Bone tissue engineering;   

Others  :  1138832
DOI  :  10.1186/s13018-014-0100-9

 received in 2014-04-13, accepted in 2014-10-08,  发布年份 2014

【 摘 要 】

Background

This study aims to investigate the biocompatibility and in vivo osteogenic capability of the novel bone tissue engineering scaffold apatite-wollastonite-magnetic glass ceramic/chitosan (A-W-MGC/CS).

Methods

Rabbit bone marrow stromal cells (BMSCs) were transfected with adenovirus-human bone morphogenetic protein-2-green fluorescent protein (Ad-hBMP2-GFP). The transfected BMSCs were then inoculated onto the scaffold material A-W-MGC/CS to construct tissue-engineered bone. The attachment and proliferation of BMSCs were observed by scanning electron microscopy (SEM) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) detection, respectively. Rabbit models of bone defects were established and divided into three groups. Experimental group 1 was implanted with prepared tissue-engineered bone. Experimental group 2 was implanted with A-W-MGC/CS without transfected BMSCs. The blank group was injected with transfected BMSCs, without implantation of any scaffold. In the 12th week after surgery, the repair of bone defect was observed by X-ray examination, and histological observations of the area of bone defect were performed.

Results

A-W-MGC/CS resulted in good BMSC attachment and had no obvious effects on cell proliferation. In experimental group 1, good repair of bone defect was observed, and the scaffold material degraded completely. In experimental group 2, new bone was formed, but its quality was poor. In the blank group, there was mainly filling of fibrous connective tissues with no observable bone defect repair.

Conclusion

A-W-MGC/CS possesses good biocompatibility and in vivo osteogenic capability for bone defect repair.

【 授权许可】

   
2014 Li et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

Figure 6.

Figure 7.

Figure 8.

Figure 9.

Figure 10.

【 参考文献 】

• [1]Pérez-Sánchez MJ, Ramírez-Glindon E, Lledó-Gil M, Calvo-Guirado JL, Pérez-Sánchez C: Biomaterials for bone regeneration. Med Oral Patol Oral Cir Bucal 2010, 15:e517-e522.
• [2]Neman J, Hambrecht A, Cadry C, Goodarzi A, Youssefzadeh J, Chen MY, Jandial R: Clinical efficacy of stem cell mediated osteogenesis and bioceramics for bone tissue engineering. Adv Exp Med Biol 2012, 760:174-187.
• [3]Porter JR, Henson A, Ryan S, Popat KC: Biocompatibility and mesenchymal stem cell response to poly (epsilon-caprolactone) nanowire surfaces for orthopedic tissue engineering. Tissue Eng Part A 2009, 15:2547-2559.
• [4]Caralla T, Joshi P, Fleury S, Luangphakdy V, Shinohara K, Pan H, Boehm C, Vasanji A, Hefferan TE, Walker E, Yaszemski M, Hascall V, Zborowski M, Muschler GF: In vivo transplantation of autogenous marrow-derived cells following rapid intraoperative magnetic separation based on hyaluronan to augment bone regeneration. Tissue Eng Part A 2013, 19:125-134.
• [5]Ciapetti G, Granchi D, Baldini N: The combined use of mesenchymal stromal cells and scaffolds for bone repair. Curr Pharm Des 2012, 18:1796-1820.
• [6]Meinel L, Hofmann S, Betz O, Fajardo R, Merkle HP, Langer R, Evans CH, Vunjak-Novakovic G, Kaplan DL: Osteogenesis by human mesenchymal stem cells cultured on silk biomaterials comparison of adenovirus mediated gene transfer and protein delivery of BMP-2. Biomaterials 2006, 27:4993-5002.
• [7]Xynos ID, Hukkanen MV, Batten JJ, Buttery LD, Hench LL, Polak JM: Bioglass 45S5 stimulates osteoblast turnover and enhances bone formation in vitro: implications and applications for bone tissue engineering. Calcif Tissue Int 2000, 67:321-329.
• [8]Neman J, Hambrecht A, Cadry C, Jandial R: Stem cell-mediated osteogenesis: therapeutic potential for bone tissue engineering. Biologics 2012, 6:47-57.
• [9]Da Li G, Da Zhou L, Pan TH, Chen GS, Lin Y, Mao M, Yan G: Effect of Mn-Zn ferrite on apatite-wollastonite glass-ceramic (A-W GC).Biomed Mater 2009, 4:045001.
• [10]Dunn CA, Jin Q, Taba M Jr, Franceschi RT, Bruce Rutherford R, Giannobile WV: BMP gene delivery for alveolar bone engineering at dental implants. Mol Ther 2005, 11:294-299.
• [11]Fujioka-Kobayashi M, Ota MS, Shimoda A, Nakahama K, Akiyoshi K, Miyamoto Y, Iseki S: Cholesteryl group- and acryloyl group-bearing pullulan nanogel to deliver BMP2 and FGF18 for bone tissue engineering. Biomaterials 2012, 33:7613-7620.
• [12]Pelled G, Snedeker JG, Ben-Arav A, Rigozzi S, Zilberman Y, Kimelman-Bleich N, Gazit Z, Müller R, Gazit D: Smad8/BMP2-engineered mesenchymal stem cells induce accelerated recovery of the biomechanical properties of the Achilles tendon. J Orthop Res 2012, 30:1932-1939.
• [13]Gassner D, Durham D, Pfannenstiel SC, Brough DE, Staecker H: Canalostomy as a surgical approach for cochlear gene therapy in the rat. Anat Rec (Hoboken) 2012, 295:1830-1836.
• [14]Menendez MI, Clark DJ, Carlton M, Flanigan DC, Jia G, Sammet S, Weisbrode SE, Knopp MV, Bertone AL: Direct delayed human adenoviral BMP-2 or BMP-6 gene therapy for bone and cartilage regeneration in a pony osteochondral model. Osteoarthritis Cartilage 2011, 19:1066-1075.
• [15]Misteli T, Spector DL: Application of the green fluorescent protein in cell biology and biotechnology. Nat Biotechnol 1997, 15:961-964.
• [16]Ye XY, Niu XM, Tang NW, Xu YH, Li ZM, Yu YF, Lu S, Chen SW: Adenovirus mediated knockdown of bone morphogenetic protein 2 inhibits human lung cancer growth and invasion in vitro and in vivo. Int J Immunopathol Pharmacol 2012, 25:967-976.
• [17]Liu D, Hu L, Zhang Z, Li QY, Wang G: Construction of human BMP2-IRES-HIF1αmu adenovirus expression vector and its expression in mesenchymal stem cells. Mol Med Report 2013, 7:659-663.
• [18]Amini AR, Laurencin CT, Nukavarapu SP: Bone tissue engineering: recent advances and challenges. Crit Rev Biomed Eng 2012, 40:363-408.
• [19]Kohgo T, Yamada Y, Ito K, Yajima A, Yoshimi R, Okabe K, Baba S, Ueda M: Bone regeneration with self-assembling peptide nanofiber scaffolds in tissue engineering for osseointegration of dental implants. Int J Periodontics Restorative Dent 2011, 31:e9-e16.
• [20]Lee SS, Huang BJ, Kaltz SR, Sur S, Newcomb CJ, Stock SR, Shah RN, Stupp SI: Bone regeneration with low dose BMP-2 amplified by biomimetic supramolecular nanofibers within collagen scaffolds. Biomaterials 2013, 34:452-459.