Genome sequence of type strain of Staphylococcus aureus subsp. aureus

Gut Pathogens

Chang-Jun Cha³  Jongsik Chun²  Hana Yi⁴  Bong-Soo Kim¹ 

[1] ChunLab, Inc., Seoul, Republic of Korea;School of Biological Sciences and Bioinformatics Institute, BIO-MAX, Seoul National University, Seoul, Republic of Korea;Department of Systems Biotechnology, Chung-Ang University, Anseong, Republic of Korea;Korea University Guro Hospital, Korea University, Seoul, Republic of Korea

关键词: Hybrid assembly;    Type strain;    Genome sequencing;    Staphylococcus aureus subsp. aureus;   

Others  :  817553
DOI  :  10.1186/1757-4749-6-6

 received in 2014-01-28, accepted in 2014-03-11,  发布年份 2014

【 摘 要 】

Background

Staphylococcus aureus is a pathogen that causes food poisoning and community-associated infection with antibiotic resistance. This species is an indigenous intestinal microbe found in infants and not found in adult intestine. The relatively small genome size and rapid evolution of antibiotic resistance genes in the species have been drawing an increasing attention in public health. To extend our understanding of the species and use the genome data for comparative genomic studies, we sequenced the type strain of S. aureus subsp. aureus DSM 20231^T.

Results

Seventeen contigs were generated using hybrid assembly of sequences derived from the Roche 454 and Illumina systems. The length of the genome sequence was 2,902,619 bases with a G + C content of 32.8%. Among the 2,550 annotated CDSs, 44 CDSs were annotated to antibiotic resistance genes and 13 CDSs were related to methicillin resistance. It is interesting to note that this strain was first isolated in 1884 before methicillin was generally used on patients.

Conclusions

The present study analyzed the genome sequence of S. aureus subsp. aureus type strain as the reference sequence for comparative genomic analyses of clinical isolates. Methicillin resistance genes found in the genome indicate the presence of antibiotic resistance mechanism prior to the usage of antibiotics. Further comparative genomic studies of methicillin-resistant strains based on this reference genome would help to understand the evolution of resistance mechanism and dissemination of resistance genes.

【 授权许可】

   
2014 Kim et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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