A proof of concept study to assess the potential of PCR testing to detect natural Mycobacterium bovis infection in South American camelids

Irish Veterinary Journal

Kirsty Line¹  Adrian McGoldrick¹  Jeremy I Chanter¹  Timothy R Crawshaw¹ 

[1] Animal Health and Veterinary Laboratories Agency Starcross, Staplake Mount, Starcross, Exeter, Devon EX6 8PE, England

关键词: Clinical samples;    Polymerase chain reaction;    Mycobacterium bovis;    Llama;    Alpaca;   

Others  :  805626
DOI  :  10.1186/2046-0481-67-5

 received in 2013-10-29, accepted in 2014-02-03,  发布年份 2014

【 摘 要 】

Background

Cases of Mycobacterium bovis infection South American camelids have been increasing in Great Britain. Current antemortem immunological tests have some limitations. Cases at post mortem examination frequently show extensive pathology. The feasibility of detecting Mycobacterium bovis DNA in clinical samples was investigated.

Findings

A sensitive extraction methodology was developed and used on nasal swabs and faeces taken post-mortem to assess the potential for a PCR test to detect Mycobacterium bovis in clinical samples. The gross pathology of the studied South American camelids was scored and a significantly greater proportion of South American camelids with more severe pathology were positive in both the nasal swab and faecal PCR tests. A combination of the nasal swab and faecal PCR tests detected 63.9% of all the South American camelids with pathology that were tested.

Conclusions

The results suggest that antemortem diagnosis of Mycobacterium bovis in South American camelids may be possible using a PCR test on clinical samples, however more work is required to determine sensitivity and specificity, and the practicalities of applying the test in the field.

【 授权许可】

   
2014 Crawshaw et al.; licensee BioMed Central Ltd.

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