Cytotoxic effects of cobalt and nickel ions on osteocytes in vitro

Journal of Orthopaedic Surgery and Research

Kotaro Sena⁴  Toyama Yoshiaki²  Nadim J Hallab³  Dale R Sumner³  Amarjit S Virdi³  Marco S Caicedo³  Vbenosawemwinghaye Orhue¹  Arihiko Kanaji² 

[1] Department of Anatomy & Cell Biology, Rush University Medical Center, 600 South Paulina Street, Armour Academic Center Suite 507, Chicago 60612, IL, USA;Department of Orthopaedic Surgery, Keio University, School of Medicine, Tokyo 160-8582, Japan;Department of Orthopedic Surgery, Rush University Medical Center, 1725 West Harrison Street, Cohn Research Building Suite 722, Chicago 60612, IL, USA;Department of Periodontology, Kagoshima University, Kagoshima 890-8544, Japan

关键词: Osteocytes;    Metal ions;    Cobalt-chromium-molybdenum;    Metal-on-metal bearing surfaces;   

Others  :  1152008
DOI  :  10.1186/s13018-014-0091-6

 received in 2013-11-20, accepted in 2014-09-24,  发布年份 2014

【 摘 要 】

Background

Metal-on-metal prostheses undergo wear and corrosion, releasing soluble ions and wear particles into the surrounding environment. Reports described early failures of the metal-on-metal prostheses, with histologic features similar to a Type IV immune response. Mechanisms by which metal wear products and metal ion causing this reaction are not completely understood, and the effects of metal ions on osteocytes, which represent more than 95% of all the bone cells, have not been also studied. We hypothesized that soluble metal ions released from the cobalt-chromium-molybdenum (Co-Cr-Mo) prosthesis may have cytotoxic effect on osteocytes.

Methods

MLO-Y4 osteocytes were treated with various metal ion solutions for 24 and 48 h. The effect of ion treatment on cytotoxicity was assessed by WST-1 reagents and cell death ELISA. Morphological changes were analyzed by a phase-contrast microscope or fluorescent microscope using Hoechst 33342 and propidium iodine staining.

Results

Cr and Mo ions did not cause cell death under 0.50 mM, highest concentration studied, whereas Co and Ni ions had significant cytotoxic effect on MLO-Y4 cells at concentrations grater than 0.10 mM and at 0.50 mM, respectively, in a dose-dependent manner. According to the ELISA data, osteocytes treated with Co ions were more susceptible to necrotic than apoptotic cell death, while Ni ions caused osteocyte apoptosis. The morphological assays show that cells treated with Co and Ni ions at high concentration were fewer in number and rounded. In addition, fluorescent images showed a marked reduction in live cells and an increase in dead osteocytes treated with Co and Ni ions at high concentration.

Conclusions

Metal ions released from metal-on-metal bearing surfaces have potentially cytotoxic effects on MLO-Y4 osteocytes, in vitro.

【 授权许可】

   
2014 Kanaji et al.; licensee BioMed Central Ltd.

【 图 表 】

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