Wound trauma alters ionizing radiation dose assessment

Cell & Bioscience

G David Ledney¹  Thomas B Elliott¹  Risaku Fukumoto¹  Lynnette H Cary¹  Patrick H Ney¹  Ian C Dews¹  Min Zhai¹  True M Burns¹  Bradley R Garrison¹  Juliann G Kiang² 

[1] Radiation Combined Injury Program, Armed Forces Radiobiology Research Institute, Bethesda, MD 20889-5603, USA;Department of Medicine, Uniformed Services University of The Health Sciences, Bethesda, MD 20814, USA

关键词: Survivin;    DNA damage;    Cytokine;    γ-H2AX;    Splenocyte;    Platelet;    Neutrophil;    Lymphocyte;    Combined injury;    Wound;    Radiation;   

Others  :  793422
DOI  :  10.1186/2045-3701-2-20

 received in 2012-05-02, accepted in 2012-06-11,  发布年份 2012

【 摘 要 】

Background

Wounding following whole-body γ-irradiation (radiation combined injury, RCI) increases mortality. Wounding-induced increases in radiation mortality are triggered by sustained activation of inducible nitric oxide synthase pathways, persistent alteration of cytokine homeostasis, and increased susceptibility to bacterial infection. Among these factors, cytokines along with other biomarkers have been adopted for biodosimetric evaluation and assessment of radiation dose and injury. Therefore, wounding could complicate biodosimetric assessments.

Results

In this report, such confounding effects were addressed. Mice were given ⁶⁰Co γ-photon radiation followed by skin wounding. Wound trauma exacerbated radiation-induced mortality, body-weight loss, and wound healing. Analyses of DNA damage in bone-marrow cells and peripheral blood mononuclear cells (PBMCs), changes in hematology and cytokine profiles, and fundamental clinical signs were evaluated. Early biomarkers (1 d after RCI) vs. irradiation alone included significant decreases in survivin expression in bone marrow cells, enhanced increases in γ-H2AX formation in Lin⁺ bone marrow cells, enhanced increases in IL-1β, IL-6, IL-8, and G-CSF concentrations in blood, and concomitant decreases in γ-H2AX formation in PBMCs and decreases in numbers of splenocytes, lymphocytes, and neutrophils. Intermediate biomarkers (7 – 10 d after RCI) included continuously decreased γ-H2AX formation in PBMC and enhanced increases in IL-1β, IL-6, IL-8, and G-CSF concentrations in blood. The clinical signs evaluated after RCI were increased water consumption, decreased body weight, and decreased wound healing rate and survival rate. Late clinical signs (30 d after RCI) included poor survival and wound healing.

Conclusion

Results suggest that confounding factors such as wounding alters ionizing radiation dose assessment and agents inhibiting these responses may prove therapeutic for radiation combined injury and reduce related mortality.

【 授权许可】

   
2012 Kiang et al.; licensee BioMed Central Ltd.

【 图 表 】

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