Protective effects of calcium gluconate on osteoarthritis induced by anterior cruciate ligament transection and partial medial meniscectomy in Sprague–Dawley rats

Journal of Orthopaedic Surgery and Research

Young-Joon Lee²  Sae-Kwang Ku¹  Ki-Young Kim³  Joo-Wan Kim³  Su-Jin Kang² 

[1] Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University, 1, Hannydaero, Gyeongsan, Gyeongsangbuk-do 712-715, Republic of Korea;Department of Preventive Medicine, College of Korean Medicine, Deagu Haany University, 1, Hannydaero, Gyeongsan, Gyeongsangbuk-do 712-715, Republic of Korea;Glucan Corporation and Marine Bio-Industry Development Center, Busan 619-912, Republic of Korea

关键词: Apoptosis;    COX-2;    Osteoarthritis;    Calcium gluconate;   

Others  :  813584
DOI  :  10.1186/1749-799X-9-14

 received in 2013-10-28, accepted in 2014-02-25,  发布年份 2014

【 摘 要 】

Background

This study aimed to determine whether calcium gluconate exerts protective effects on osteoarthritis (OA) induced by anterior cruciate ligament (ACL) transection and partial medial meniscectomy.

Methods

Calcium gluconate was administered by mouth daily for 84 days to male ACL transected and partial medial meniscectomized Sprague–Dawley rats 1 week after operation.

Results

Eighty-four days of treatment with 50 mg/kg calcium gluconate led to a lower degree of articular stiffness and cartilage damage compared to the OA control, possibly through inhibition of overexpressed cyclooxygenase (COX)-2 and related chondrocyte apoptosis. Similar favorable effects on stiffness and cartilage were detected in calcium gluconate-administered rats. Additionally, calcium gluconate increased 5-bromo-2′-deoxyuridine (BrdU) uptake based on observation of BrdU-immunoreactive cells on both the femur and tibia articular surface cartilages 84 days after intra-joint treatment with calcium gluconate.

Conclusions

Taken together, our results demonstrate that calcium gluconate has a protective effect against OA through inhibition of COX-2 and related chondrocyte apoptosis.

【 授权许可】

   
2014 Kang et al.; licensee BioMed Central Ltd.

【 图 表 】

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