Surface plasmon resonance based on molecularly imprinted nanoparticles for the picomolar detection of the iron regulating hormone Hepcidin-25

Journal of Nanobiotechnology

Alessandra Maria Bossi¹  Domenico Girelli³  Annalisa Castagna³  Mirko Busato¹  Erica Iacob²  Mario Barozzi²  Michele Bovi¹  Ambra Vestri¹  Erika Andreetto¹  Lucia Cenci¹ 

[1] Department of Biotechnology, University of Verona, Strada Le Grazie 15, Verona, 37134, Italy;Center for Materials and Microsystems CMM-MNF, FBK Fondazione Bruno Kessler, Via Sommarive 18, Povo-Trento, 38123, Italy;Department of Medicine, University of Verona, Section of Internal Medicine B, Verona, 37134, Italy

关键词: Iron metabolism;    Surface plasmon resonance;    Biosensor;    Hepcidin;    Nanoparticles;    Molecularly imprinted polymers;   

Others  :  1224845
DOI  :  10.1186/s12951-015-0115-3

 received in 2014-11-05, accepted in 2015-08-10,  发布年份 2015

【 摘 要 】

Background

Molecularly imprinted polymer (MIP) technique is a powerful mean to produce tailor made synthetic recognition sites. Here precipitation polymerization was exploited to produce a library of MIP nanoparticles (NPs) targeting the N terminus of the hormone Hepcidin-25, whose serum levels correlate with iron dis-metabolisms and doping. Biotinylated MIP NPs were immobilized to NeutrAvidin™ SPR sensor chip. The response of the MIP NP sensor to Hepcidin-25 was studied.

Findings

Morphological analysis showed MIP NPs of 20–50 nm; MIP NP exhibited high affinity and selectivity for the target analyte: low nanomolar Kds for the interaction NP/Hepcidin-25, but none for the NP/non regulative Hepcidin-20. The MIP NP were integrated as recognition element in SPR allowing the detection of Hepcidin-25 in 3 min. Linearity was observed with the logarithm of Hepcidin-25 concentration in the range 7.2–720 pM. LOD was 5 pM. The response for Hepcidin-20 was limited. Hepcidin-25 determination in real serum samples spiked with known analyte concentrations was also attempted.

Conclusion

The integration of MIP NP to SPR allowed the determination of Hepcidin-25 at picomolar concentrations in short times outperforming the actual state of art. Optimization is still needed for real sample measurements in view of future clinical applications.

【 授权许可】

   
2015 Cenci et al.

【 图 表 】

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