A novel approach for pathogen reduction in wastewater treatment

Journal of Environmental Health Science Engineering

Anusuya Sundaram¹  Dhevagi Periasamy¹ 

[1] Department of Environmental Sciences, Directorate of Natural Resources Management, Tamil Nadu Agricultural University, 641003, Tamil Nadu, India

关键词: Wastewater treatment;    Specific phage;    Pathogen reduction;    Hospital wastewater;   

Others  :  820643
DOI  :  10.1186/2052-336X-11-12

 received in 2012-09-21, accepted in 2013-06-19,  发布年份 2013

【 摘 要 】

Many sewage waste treatment systems are aiming for complete pathogen removal which necessitates search for novel approaches that does not harm the environment. One such novel approach is exploring the possibilities of bacteriophages for pathogen removal. Hospital wastewater was collected from different locations of Tamil Nadu and used for the study. The total heterotroph and total coliform population ranged from 1.6 × 10⁵ to 8.3 × 10⁶ per mL and from 1.2 × 10³ to 1.6 × 10³/ 100 mL of sample respectively. Higher frequency of antibiotic resistant E. coli, Pseudomonas sp. Streptococcus sp and Bacillus spp were observed in all the places, which clearly indicated the extent of pollution. All the samples had specific phages against E. coli and none of the samples had phages against MTCC culture. E. coli specific phage was isolated and the population of phage required for effective killing of E. coli has been standardized as 3 × 10⁴ pfu / mL of lysate. The inoculation resulted in 100% removal of pathogen from sewage water within 14 hours of incubation.

【 授权许可】

   
2013 Periasamy and Sundaram; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Saha MR, Dutta P, Bhattacharya SK Rasaily R, Mitra U, Dutta D, Bhattacharya MK, Pal SC: Multidrug resistant bacteria. Indian J Med Res 1992, 95:179-180.
• [2]Chitnis V, Chitnis D, Patil S, Ravi K: Hospital effluent: a source of multiple drug resistant bacteria. Curr Sci 2002, 79:989-991.
• [3]Ekhaise FO, Omavwaya BP: Influence of hospital wastewater discharged from University Benin Teaching Hospital (UBTH), Benin City on its receiving environment. J Agric and Environ Sci 2008, 4:484-488.
• [4]Thiel K: Old Dogma, New tricks – 21st Century phage therapy. Nat Biotechnol 2004, 22:31-36.
• [5]APHA: Standard methods for estimation of water and wastewater. 16th edition. Washington DC: American Public Health Association; 1989.
• [6]Cappuccino JG, Sherman N: Microbiology a Laboratory Manual. Sixth edition. Singapore: Pvt. Ltd. Indian Branch; 2002.
• [7]Holt GJ, Krieg RN, Sneath AHP, Stately TJ, Williams TS: Bergey’s manual of Determinative Bacteriology. 9th edition. Lippincott; 1994.
• [8]Johnson TR, Case CL: Laboratory Experiments in Microbiology. New Jersey: TheBenjamin / Cummings publishing company, Inc; 1995:125-129.
• [9]Bradshaw LJ: Laboratory Microbiology. Third edition. USA: W. B. Saunders company Press; 1979:343.
• [10]Sambrook J, Russell RWD: Molecular cloning. In A laboratory manual. Cold spring harbor, NY: Cold spring harbor laboratory press; 1991:2.25-2.33.
• [11]Zoeteman BCG: Sensory assessment of water quality. UK: U.K.: Oxford Pergaman press; 1980.
• [12]Sangu RPS, Sharma SK: An assessment of water quality of river Ganga at Garmukeshwar. Ind J Ecol 1987, 14(20):278-287.
• [13]Subba Rao C, Subba Rao NV: Ground water quality in residential colony. Ind J Environ Hlth 1995, 37(4):295-300.
• [14]Yanggen DA, Born SM: Protecting ground water quality by managing local land use. J Soil Water Conser 1990, 45(2):207-210.
• [15]Tyagi P, Buddi D, Chowdary R, Sawhney R: Physicochemical quality of ground water in industrial areas of India. Pollut Res 2000, 19:443-445.
• [16]Dasgupta A, Purohit KM: Status of the surface water quality of Mandiakudar. Pollut Res 2001, 20(1):103-110.
• [17]Aluyi ASA, Ekhaise OF, Adelusi MD: Effect of human activities and oil pollution on the microbiological and physiological quality of Udu River, Warri Nigeria. J Appl Sci 2006, 6(5):1214-1219.
• [18]Rajurkar NS, Nongbi B, Patwardhan AM: Physicochemical and microbial analysis of Umian(Brapani) lake water. Ind J Environ Protec 2003, 23:6.
• [19]Joshi VA, Manivel V, Ravindra R, Kelkar PS: Water quality assessment in Ramanathapuram district. Ind J Environ Protec 2002, 22(9):970-977.
• [20]Moharir A, Ramteke DS, Moghe CA, Wate SR, Sarin R: Surface and ground water quality assessment in Bina region. Ind J Environ Protec 2002, 22(9):961-969.
• [21]Bauer AW, Perry DM, Kirby WM: Single-disk antibiotic-sensitivity testing of staphylococci; an analysis of technique and results. AMA Arch Intern Med 1959, 104(2):208-216.
• [22]Neema S, Premchandani P, Asolkar MV, Chitnis DS: Indian J Med Sci. 1997, 51:275-280.
• [23]Rangnekar VM, Mukerji S, Gadre SV, Chitnis DS: Prevalence of multidrug resistant coliforms in hman gut: Effect of antibiotic therapy. Bull Haffkine Inst 1981, 9:8-14.
• [24]Sulak Velidze AZ, Alavidze M: Bacteriophage therapy. Antimicrob Agents Chemother 2001, 45(3):649-659.
• [25]Marks T: Bacteriophages and biotechnology: a review. J Chem tech Biotechnol 2000, 75:6-17.
• [26]Maloy SR, Cronana JE, David F: Genetics of phage T4. In Microbial Genetics. New Delhi: Narosa Publishing House; 2008:346-370.
• [27]Shuttle CA: Theoritical, evolutionary and geochemical consequences of viral infection of cyanobacteria and eukaryotic algae. In Viral Ecology. Edited by Horst CJ. USA: Academic; 2000.
• [28]Alonso MD, Rodriguez J, Borrego JJ: Characterization of marine bacteriophages isolated from Alboran sea (Western Mediterranean). J Plankton Res 2002, 24:1079-1087.
• [29]Hennes KP, Simon M: Significance of bacteriophages for controlling bacterioplankton growth in a mesotrophic lake. Appl Environ Microbiol 2005, 61:333-340.
• [30]Payne RJH, Jansen VAA: Understanding phage therapy as a density dependent kinetic process. J Theor Biol 2001, 208:37-48.
• [31]Connon SA, Giovannoni SJ: High –throughput methods for culturing microorganisms in very low nutrient media yield diverse new marine isolates. Appl Environ Microbiol 2002, 68:3878-3885.
• [32]Rappe MS, Connon SA, Vergin KL, Giovannoni SJ: Cultivation of the ubiquitous SARII marine bacterioplankton clade. Nature 2002, 418:633-639.
• [33]Sullivan OLA, Fuller KE, Thomas EM, Turley CM, Fry JC, Weightman AJ: Distribution and culturability of the uncultivated ‘AGG58 cluster’ of the Bacteriodes phylum in aquatic environments. FEMS Microbial Ecol 2004, 47:359-370.
• [34]Hantula J, Kurki A, Vuoriranta P, Bamford B: Ecology of bacteriophages infecting activated sludge bacteria. Appl Environ Microbiol 1991, 57:2147-2151.
• [35]Jensen EC, Sehrader HS, Rieland B, Thompson TL, Lee KW, Nickers KW, Kokjon TA: Prevalence of broad host range lytic bacteriophages of Spharotilus natans, Escherichia coli and Pseudomonas aeruginosa. Appl Environ Microbial 1998, 64:575-580.
• [36]Harries DL: Reduction of Salmonella by bacteriophage treatment. USA: Research Report by National Park Board; 2000.
• [37]Thomus L, Jungschaffer G, Sprossler B: Improved sludge dewatering by enzymatic treatment. Wat Sci Tech 1993, 28:189-192.
• [38]Wolf A, Wiese J, Jost G, Witzel K: Wide geographic distributions of bacteriophages that lyse the same indigenous fresh water isolate (Sphingomonas sp.StrainB18). Appl Environ Microbiol 2003, 69:2395-2398.
• [39]Tanji Y, Mizoguchi K, Yoichi M, Morita M, Hori K, Unno H: Fate of coliphage in a wastewater treatment. J Biosci Bioeng 2002, 94:172-174.
• [40]Kim TD, Unno H: The role of microbes in the removal and inactivation of viruses in a biological wastewater treatment system. Wat Sci Tech 1996, 33:243-250.