Characterization of vascular endothelial progenitor cells from chicken bone marrow

BMC Veterinary Research

Yuehui Ma¹  Weijun Guan¹  Yabin Pu¹  Minghai Zhang³  Lingling Hou²  Chunyu Bai³ 

[1] Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China;College of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, 100044, China;College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, China

关键词: Isolation;    Endothelial progenitor cells;    Biological characteristics;    Chicken;   

Others  :  1119833
DOI  :  10.1186/1746-6148-8-54

 received in 2011-10-04, accepted in 2012-05-14,  发布年份 2012

【 摘 要 】

Background

Endothelial progenitor cells (EPC) are a type of stem cell used in the treatment of atherosclerosis, vascular injury and regeneration. At present, most of the EPCs studied are from human and mouse, whereas the study of poultry-derived EPCs has rarely been reported. In the present study, chicken bone marrow-derived EPCs were isolated and studied at the cellular level using immunofluorescence and RT-PCR.

Results

We found that the majority of chicken EPCs were spindle shaped. The growth-curves of chicken EPCs at passages (P) 1, -5 and -9 were typically “S”-shaped. The viability of chicken EPCs, before and after cryopreservation was 92.2% and 81.1%, respectively. Thus, cryopreservation had no obvious effects on the viability of chicken EPCs. Dil-ac-LDL and FITC-UAE-1 uptake assays and immunofluorescent detection of the cell surface markers CD34, CD133, VEGFR-2 confirmed that the cells obtained in vitro were EPCs. Observation of endothelial-specific Weibel-Palade bodies using transmission electron microscopy further confirmed that the cells were of endothelial lineage. In addition, chicken EPCs differentiated into endothelial cells and smooth muscle cells upon induction with VEGF and PDGF-BB, respectively, suggesting that the chicken EPCs retained multipotency in vitro.

Conclusions

These results suggest that chicken EPCs not only have strong self-renewal capacity, but also the potential to differentiate into endothelial and smooth muscle cells. This research provides theoretical basis and experimental evidence for potential therapeutic application of endothelial progenitor cells in the treatment of atherosclerosis, vascular injury and diabetic complications.

【 授权许可】

   
2012 Bai et al.; licensee BioMed Central Ltd.

【 图 表 】

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