No evidence for association between SLC11A1 and visceral leishmaniasis in India

BMC Medical Genetics

Jenefer M Blackwell¹  Shyam Sundar²  Madhukar Rai²  Kumarasamy Thangaraj³  Deepa Selvi Rani³  Michaela Fakiola¹  Sarra E Jamieson⁴  Puja Tiwary²  Medhavi Sudharshan²  Anshuman Mishra²  Joyce Oommen⁴  Sanjana Mehrotra⁴ 

[1] Cambridge Institute for Medical Research and Department of Medicine, University of Cambridge School of Clinical Medicine, Cambridge, UK;Institute of Medical Sciences, Banaras Hindu University, Varanasi, OS 221 005, India;Centre for Cellular and Molecular Biology, Hyderabad, India;Telethon Institute for Child Health Research, Centre for Child Health Research, The University of Western Australia, Subiaco, Western Australia, Australia

关键词: genetic susceptibility;    visceral leishmaniasis;    SLC11A1;   

Others  :  1178039
DOI  :  10.1186/1471-2350-12-71

 received in 2011-02-22, accepted in 2011-05-20,  发布年份 2011

【 摘 要 】

Background

SLC11A1 has pleiotropic effects on macrophage function and remains a strong candidate for infectious disease susceptibility. 5' and/or 3' polymorphisms have been associated with tuberculosis, leprosy, and visceral leishmaniasis (VL). Most studies undertaken to date were under-powered, and none has been replicated within a population. Association with tuberculosis has replicated variably across populations. Here we investigate SLC11A1 and VL in India.

Methods

Nine polymorphisms (rs34448891, rs7573065, rs2276631, rs3731865, rs17221959, rs2279015, rs17235409, rs17235416, rs17229009) that tag linkage disequilibrium blocks across SLC11A1 were genotyped in primary family-based (313 cases; 176 families) and replication (941 cases; 992 controls) samples. Family- and population-based analyses were performed to look for association between SLC11A1 variants and VL. Quantitative RT/PCR was used to compare SLC11A1 expression in mRNA from paired splenic aspirates taken before and after treatment from 24 VL patients carrying different genotypes at the functional promoter GT_n polymorphism (rs34448891).

Results

No associations were observed between VL and polymorphisms at SLC11A1 that were either robust to correction for multiple testing or replicated across primary and replication samples. No differences in expression of SLC11A1 were observed when comparing pre- and post-treatment samples, or between individuals carrying different genotypes at the GT_n repeat.

Conclusions

This is the first well-powered study of SLC11A1 as a candidate for VL, which we conclude does not have a major role in regulating VL susceptibility in India.

【 授权许可】

   
2011 Mehrotra et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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