Neuroprotective effects of Liriope platyphylla extract against hydrogen peroxide-induced cytotoxicity in human neuroblastoma SH-SY5Y cells

BMC Complementary and Alternative Medicine

Jin Yeul Ma¹  Won-Kyung Cho¹  Jong Wook Jeon¹  Hwayong Park¹  Heeeun Lee¹  Hee Ra Park¹ 

[1] Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM), 70, Cheomdan-ro, Daegu 701-300, Dong-gu, South Korea

关键词: Antiapoptotic effect;    Antioxidant activity;    Neuroprotective effects;    Liriope platyphylla;   

Others  :  1212020
DOI  :  10.1186/s12906-015-0679-3

 received in 2015-03-17, accepted in 2015-05-19,  发布年份 2015

【 摘 要 】

Background

Oxidative stress is involved in neuronal cell death and mitochondrial dysfunction in neurodegenerative diseases. Liriope platyphylla (LP) has been suggested to have anti-inflammation, anti-bacterial, and anti-cancer effects. However, whether LP exerts neuroprotective effects on neuronal cells is unknown.

Methods

The present study was performed to investigate the neuroprotective effects of LP extract (LPE) against hydrogen peroxide (H₂O₂)-induced injury in human neuroblastoma cells SH-SY5Y. To test neuroprotective effects of LPE, we performed cell viability assay, flow cytometry analysis and western blot analysis. In addition, mitochondrial membrane potential (MMP) and oxidative stress were performed to evaluate the anti-apoptotic and anti-oxidant effects.

Results

LPE pretreatment conferred significant protection against the H₂O₂-induced decrease of SH-SY5Y cell viability. H₂O₂-induced increases of intracellular oxidative stress and mitochondrial dysfunction were attenuated by LPE pretreatment. Therefore, LPE pretreatment prevented SH-SY5Y cell injury. Treatment with H₂O₂ significantly induced poly(ADP ribose) polymerase (PARP) and caspase-3 cleavage, which was blocked by LPE. We found that p38 activation was involved in the neuroprotective effects of LPE.

Conclusions

Current findings suggest that LPE exerts neuroprotective effects against H₂O₂-induced apoptotic cell death by modulating p38 activation in SH-SY5Y cells. Therefore, LPE has potential anti-apoptotic effects that may be neuroprotective in neurodegenerative diseases and aging-related dementia.

【 授权许可】

   
2015 Park et al.

【 图 表 】

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