Anti-Neuroinflammatory and Neurotrophic Effects of Combined Therapy with Annexin II and Reg-2 on Injured Spinal Cord

Neuro-signals

关键词: Annexin II;    Reg-2 protein;    Spinal cord injury;    White matter sparing;    Neuroprotective effects;   

DOI  :  10.1159/000324272

学科分类：神经科学

来源: S Karger AG

【 摘 要 】

The present study was designed to investigate the neuroprotective effects of Ca²⁺-dependent phospholipid-binding protein annexin II and a secreted protein Reg-2 (regeneration gene protein 2) in spinal cord injury (SCI) model produced by contusion SCI at T₉ using the weight drop method. The agents were delivered intrathecally with Alzet miniosmotic pumps. We found that annexin II and Reg-2 remarkably reduced neuronal death, attenuated tissue damage and alleviated detrimental inflammation in vivo; meanwhile, a significant increase in white matter sparing and myelination area was observed. The propriospinal axons and long-distance supraspinal pathways were protected by the treatments as revealed by retrograde tracing. Basso Beattie Bresnahan locomotor rating scores also revealed a measurable behavioral improvement. However, no evident behavioral improvements in locomotor performance were achieved by the combined treatment with annexin II and Reg-2, compared with the separate treatment with annexin II and Reg-2.

【 授权许可】

CC BY-NC-ND