Liver transcriptome analysis in gilthead sea bream upon exposure to low temperature

BMC Genomics

Tomaso Patarnello³  Luca Bargelloni³  Chiara Romualdi¹  Silvia Livi⁴  Giovanna Marino⁴  Patrizia Di Marco⁴  Tommaso Petochi⁴  Serena Ferraresso³  Massimo Milan³  Alba N Mininni² 

[1] Department of Biology, University of Padova, via G. Colombo 3, 35131 Padova, Italy;Department of European and Mediterranean Cultures, Architecture, Environment and Cultural Heritage, University of Basilicata, 75100 Matera, Italy;Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell’Università 16, 35020 Legnaro, Italy;ISPRA - Italian Institute for Environmental Protection and Research, 00144 Rome, Italy

关键词: Liver;    Gene expression;    Microarray;    Cold stress;    Winter syndrome;    Gilthead sea bream;   

Others  :  1140807
DOI  :  10.1186/1471-2164-15-765

 received in 2014-04-03, accepted in 2014-09-03,  发布年份 2014

【 摘 要 】

Background

Water temperature greatly influences the physiology and behaviour of teleost fish as other aquatic organisms. While fish are able to cope with seasonal temperature variations, thermal excursions outside their normal thermal range might exceed their ability to respond leading to severe diseases and death.

Profound differences exist in thermal tolerance across fish species living in the same geographical areas, promoting for investigating the molecular mechanisms involved in susceptibility and resistance to low and high temperatures toward a better understanding of adaptation to environmental challenges. The gilthead sea bream, Sparus aurata, is particularly sensitive to cold and the prolonged exposure to low temperatures may lead to the "winter disease", a metabolic disorder that significantly affects the aquaculture productions along the Northern Mediterranean coasts during winter-spring season. While sea bream susceptibility to low temperatures has been extensively investigated, the cascade of molecular events under such stressful condition is not fully elucidated.

Results

In the present study two groups of wild sea bream were exposed for 21 days to two temperature regimes: 16 ± 0.3°C (control group) and 6.8 ± 0.3°C (cold-exposed group) and DNA microarray analysis of liver transcriptome was carried out at different time points during cold exposure.

A large set of genes was found to be differentially expressed upon cold-exposure with increasingly relevant effects being observed after three weeks at low temperature. All major known responses to cold (i.e. anti-oxidant response, increased mitochondrial function, membrane compositional changes) were found to be conserved in the gilthead sea bream, while, evidence for a key role of unfolded protein response (UPR) to endoplasmic reticulum (ER) stress, during short- and long-term exposure to cold is reported here for the first time.

Conclusions

Transcriptome data suggest a scenario where oxidative stress, altered lipid metabolism, ATP depletion and protein denaturation converge to induce ER stress. The resulting UPR activation further promotes conditions for cell damage, and the inability to resolve ER stress leads to severe liver dysfunction and potentially to death.

【 授权许可】

   
2014 Mininni et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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