Comparative genome analysis of an avirulent and two virulent strains of avian Pasteurella multocida reveals candidate genes involved in fitness and pathogenicity

BMC Microbiology

Robert E Briggs²  Samuel K Maheswaran¹  Fred M Tatum²  Melissa Hauglund²  Samuel S Hunter³  Juan E Abrahante¹  Timothy J Johnson¹ 

[1] Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, Minnesota, USA;National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, Iowa, USA;Institute for Bioinformatics and Evolutionary Studies, University of Idaho, Moscow, Idaho, USA

关键词: Pathogenicity;    Fowl cholera;    Fitness;    Avian;    Virulence;    Genome;    Genomics;    Pasteurella multocida;   

Others  :  1143721
DOI  :  10.1186/1471-2180-13-106

 received in 2013-01-02, accepted in 2013-05-06,  发布年份 2013

【 摘 要 】

Background

Pasteurella multocida is the etiologic agent of fowl cholera, a highly contagious and severe disease of poultry causing significant mortality and morbidity throughout the world. All types of poultry are susceptible to fowl cholera. Turkeys are most susceptible to the peracute/acute forms of the disease while chickens are most susceptible to the acute and chronic forms of the disease. The whole genome of the Pm70 strain of P. multocida was sequenced and annotated in 2001. The Pm70 strain is not virulent to chickens and turkeys. In contrast, strains X73 and P1059 are highly virulent to turkeys, chickens, and other poultry species. In this study, we sequenced the genomes of P. multocida strains X73 and P1059 and undertook a detailed comparative genome analysis with the avirulent Pm70 strain. The goal of this study was to identify candidate genes in the virulent strains that may be involved in pathogenicity of fowl cholera disease.

Results

Comparison of virulent versus avirulent avian P. multocida genomes revealed 336 unique genes among the P1059 and/or X73 genomes compared to strain Pm70. Genes of interest within this subset included those encoding an L-fucose transport and utilization system, several novel sugar transport systems, and several novel hemagglutinins including one designated PfhB4. Additionally, substantial amino acid variation was observed in many core outer membrane proteins and single nucleotide polymorphism analysis confirmed a higher dN/dS ratio within proteins localized to the outer membrane.

Conclusions

Comparative analyses of highly virulent versus avirulent avian P. multocida identified a number of genomic differences that may shed light on the ability of highly virulent strains to cause disease in the avian host, including those that could be associated with enhanced virulence or fitness.

【 授权许可】

   
2013 Johnson et al.; licensee BioMed Central Ltd.

【 图 表 】

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