Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium

BMC Microbiology

Douwe van Sinderen¹  Marco Ventura³  Lokesh Joshi⁴  Marian Kane⁴  Michelle Kilcoyne²  Mary O’Connell Motherway¹  Muireann Egan¹ 

[1] School of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland;Microbiology, School of Natural Sciences, National University of Ireland, Galway, Ireland;Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy;Glycoscience Group, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland

关键词: Galactose;    Sialic acid;    Fucose;    Probiotic;    Bifidobacteria;   

Others  :  1137727
DOI  :  10.1186/s12866-014-0282-7

 received in 2014-08-08, accepted in 2014-11-03,  发布年份 2014

【 摘 要 】

Background

Bifidobacteria constitute a specific group of commensal bacteria that commonly inhabit the mammalian gastrointestinal tract. Bifidobacterium breve UCC2003 was previously shown to utilize a variety of plant/diet/host-derived carbohydrates, including cellodextrin, starch and galactan, as well as the mucin and HMO-derived monosaccharide, sialic acid. In the current study, we investigated the ability of this strain to utilize parts of a host-derived source of carbohydrate, namely the mucin glycoprotein, when grown in co-culture with the mucin-degrading Bifidobacterium bifidum PRL2010.

Results

B. breve UCC2003 was shown to exhibit growth properties in a mucin-based medium, but only when grown in the presence of B. bifidum PRL2010, which is known to metabolize mucin. A combination of HPAEC-PAD and transcriptome analyses identified some of the possible monosaccharides and oligosaccharides which support this enhanced co-cultivation growth/viability phenotype.

Conclusion

This study describes the potential existence of a gut commensal relationship between two bifidobacterial species. We demonstrate the in vitro ability of B. breve UCC2003 to cross-feed on sugars released by the mucin-degrading activity of B. bifidum PRL2010, thus advancing our knowledge on the metabolic adaptability which allows the former strain to colonize the (infant) gut by its extensive metabolic abilities to (co-)utilize available carbohydrate sources.

【 授权许可】

   
2014 Egan et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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