Acute oral toxicity of Insampaedok-san, a traditional herbal formula, in rats and its protective effects against ovalbumin-induced asthma via anti-inflammatory and antioxidant properties

BMC Complementary and Alternative Medicine

Hyeun-Kyoo Shin¹  Woo-Young Jeon¹  Ohn-Soon Kim¹  Mee-Young Lee¹  Yeji Kim¹ 

[1]Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 483 Expo-ro, Yusung-gu, Daejeon 305-811, Republic of Korea

关键词: Asthma;    Free radical scavenging;    Cytokine;    Safety;    Insampaedok-san;   

Others  :  1086479
DOI  :  10.1186/1472-6882-14-365

 received in 2013-12-06, accepted in 2014-09-24,  发布年份 2014

【 摘 要 】

Background

Insampaedok-san (ren-shen-bai-du-san in Chinese) is a traditional herbal formula widely used for the treatment of respiratory diseases in Korea and China. In this study, we investigated the acute oral toxicity of an Insampaedok-san water extract (ISSE) in rats and the antiasthmatic effects of ISSE and its mechanism in a model of asthma induced by ovalbumin (OVA) in mice.

Methods

In a safety study, ISSE was administrated orally to rats of both sexes at single doses of 0 and 5000 mg/kg. We observed body weight changes, mortality, clinical signs, and gross pathological findings. In vitro antioxidant activity of ISSE was measured using 2,2-diphenyl-2-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical scavenging methods. A model of asthma was established in mice by sensitization and challenge with OVA. We assessed the levels of type 2 T-helper cytokines, chemokines, and immunoglobulin levels, using enzyme-linked immunosorbent assays, and superoxide dismutase (SOD) activity using a kit.

Results

No adverse effects were observed in the acute ISSE toxicity study. ISSE showed potent free radical scavenging activity and inhibited the recruitment of inflammatory cells into the lung and mucus hypersecretion in OVA-challenged mice. ISSE significantly decreased levels of interleukin (IL)-4, IL-5, eotaxin, and OVA-specific immunoglobulin (Ig)E, and increased SOD activity.

Conclusions

These results indicate that ISSE is safe for human consumption and its antiasthmatic effect is associated with the ability of ISSE to attenuate inflammation and oxidative stress.

【 授权许可】

   
2014 Kim et al.; licensee BioMed Central Ltd.

【 图 表 】

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