In vitro anticoagulant and antioxidant activities of Jatropha gossypiifolia L. (Euphorbiaceae) leaves aiming therapeutical applications

BMC Complementary and Alternative Medicine

Matheus de Freitas Fernandes-Pedrosa⁴  Hugo Alexandre Oliveira Rocha²  Silvana Maria Zucolotto³  Ivanise Marina Moretti Rebecchi¹  Arnóbio Antônio Silva-Júnior⁴  Bárbara Cabral³  Rafael Barros Gomes Camara²  Thiago Souza⁴  Juliana Félix-Silva⁴ 

[1] Laboratório de Hematologia Clínica, Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Rua Gal. Gustavo Cordeiro de Farias, s/n, Petrópolis, CEP 59012-570 Natal, RN, Brazil;Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Campus Universitário, 3000, Lagoa Nova, CEP 59072-970 Natal, RN, Brazil;Laboratório de Farmacognosia, Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Rua Gal. Gustavo Cordeiro de Farias, s/n, Petrópolis, CEP 59012-570 Natal, RN, Brazil;Laboratório de Tecnologia & Biotecnologia Farmacêutica (TecBioFar), Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Rua Gal. Gustavo Cordeiro de Farias, s/n, Petrópolis, CEP 59012-570 Natal, RN, Brazil

关键词: Thin layer chromatography;    Fibrinolytic;    Cytotoxicity;    Antioxidant;    Anticoagulant;    "Bellyache bush";    "Pinhão-roxo";    Euphorbiaceae;    Jatropha gossypiifolia;   

Others  :  1085927
DOI  :  10.1186/1472-6882-14-405

 received in 2014-05-14, accepted in 2014-10-06,  发布年份 2014

【 摘 要 】

Background

Jatropha gossypiifolia L. (Euphorbiaceae) is a medicinal plant largely used in folk medicine. Teas from the leaves are popularly used as an antithrombotic agent and the branches are frequently employed as a "thick blood" agent. Considering that the anticoagulant activity associated with antioxidant properties could be beneficial for various cardiovascular diseases, this study’s aim is the evaluation of anticoagulant and antioxidant activities of J. gossypiifolia leaves, seeking new therapeutic purposes for this plant.

Methods

The aqueous leaf crude extract (CE) was prepared by decoction and was fractionated by liquid-liquid partition with solvents of increasing polarity. The phytochemical analysis was performed by thin layer chromatography (TLC) and by the spectrophotometric quantification of sugars, proteins and phenolic compounds. The anticoagulant activity was evaluated by prothrombin time (PT) and activated partial thromboplastin time (aPTT) tests. The capacity to act in the fibrinolytic system (fibrinolytic and fibrinogenolytic activities) was also assessed. The antioxidant activity was evaluated by total antioxidant capacity, reducing power, copper chelating activity, iron chelating activity, hydroxyl radical scavenging activity and superoxide radical scavenging assays. The potential toxicity was evaluated using hemolytic assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay on HEK-293 cells.

Results

CE showed significant anticoagulant activity in aPTT test, while no action was observed in PT test, suggesting a preferential action toward the intrinsic and/or common pathway of coagulation. No effect was observed in the fibrinolytic system. Using the aPTT test, it was observed that the residual aqueous (RA) fraction was the most active, being two times more active than CE. RA presented very significant antioxidant activity in all models tested comparable to or even higher than CE. Regarding the safety, CE and RA did not produce significant cytotoxicity in both tests employed. Phytochemical analysis revealed the presence of alkaloids, flavonoids, proteins, tannins, steroids and/or terpenoids and sugars.

Conclusions

CE and RA possessed significant anticoagulant and antioxidant activity and absence of cytotoxic effect in vitro, thus showing the potential of the plant, especially RA fraction, as a new source of bioactive molecules for therapeutic purposes, with particular emphasis on the treatment of cardiovascular diseases.

【 授权许可】

   
2014 Félix-Silva et al.; licensee BioMed Central Ltd.

【 图 表 】

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