Antioxidant activity of herbaceous plant extracts protect against hydrogenperoxide-induced DNA damage in human lymphocytes

BMC Research Notes

Pi-Yu Chao⁵  Hwei-Shen Lin²  Kuang-Chuan Liu¹  Hsiao-Feng Lo⁴  Meng-Yuan Huang³  Chi-Ming Yang³  Yan-Yin Yang²  Kuan-Hung Lin⁶ 

[1] Taoyuan District Agricultural Research and Extension Station, Taoyuan 327Taiwan;Graduate Institute of Applied Science of Living, Chinese Culture University,Taipei 11114, Taiwan;Research Center for Biodiversity, Academia Sinica, Nankang, Taipei 11106,Taiwan;Department of Horticulture and Landscape Architecture, National TaiwanUniversity, Taipei 11111, Taiwan;Department of Nutrition and Health Sciences, Chinese Culture University,Taipei 11114, Taiwan;Graduate Institute of Biotechnology, Chinese Culture University, Taipei11114, Taiwan

关键词: Human lymphocytes;    Comet assay;    Flavonol;    Antioxidant activity;    Herbaceous plants;   

Others  :  1232494
DOI  :  10.1186/1756-0500-6-490

 received in 2013-08-19, accepted in 2013-11-23,  发布年份 2013

【 摘 要 】

Background

Herbaceous plants containing antioxidants can protect against DNA damage. The purpose of this study was to evaluate the antioxidant substances, antioxidant activity, and protection of DNA from oxidative damage in human lymphocytes induced by hydrogen peroxide (H₂O₂). Our methods used acidic methanol and water extractions from six herbaceous plants, including Bidens alba (BA), Lycium chinense (LC), Mentha arvensis (MA), Plantago asiatica (PA), Houttuynia cordata (HC), and Centella asiatica (CA).

Methods

Antioxidant compounds such as flavonol and polyphenol were analyzed. Antioxidant activity was determined by the inhibition percentage of conjugated diene formation in a linoleic acid emulsion system and by trolox-equivalent antioxidant capacity (TEAC) assay. Their antioxidative capacities for protecting human lymphocyte DNA from H₂O₂-induced strand breaks was evaluated by comet assay.

Results

The studied plants were found to be rich in flavonols, especially myricetin in BA, morin in MA, quercetin in HC, and kaemperol in CA. In addition, polyphenol abounded in BA and CA. The best conjugated diene formation inhibition percentage was found in the acidic methanolic extract of PA. Regarding TEAC, the best antioxidant activity was generated from the acidic methanolic extract of HC. Water and acidic methanolic extracts of MA and HC both had better inhibition percentages of tail DNA% and tail moment as compared to the rest of the tested extracts, and significantly suppressed oxidative damage to lymphocyte DNA.

Conclusion

Quercetin and morin are important for preventing peroxidation and oxidative damage to DNA, and the leaves of MA and HC extracts may have excellent potential as functional ingredients representing potential sources of natural antioxidants.

【 授权许可】

   
2013 Lin et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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