Peroxynitrite decomposition catalyst prevents apoptotic cell death in a human astrocytoma cell line incubated with supernatants of HIV-infected macrophages

BMC Neuroscience

Vincenzo Mollace¹  Stefano Aquaro⁴  Carlo Federico Perno⁵  Domenicantonio Rotiroti¹  Antonio Cufari¹  Ernesto Palma¹  Michelangelo Iannone³  Donatella Paolino²  Daniela Salvemini⁶  Carolina Muscoli¹ 

[1] Faculty of Pharmacy, University of Catanzaro "Magna Graecia", Roccelletta di Borgia, Catanzaro, Italy;Department of Pharmaceutical Sciences, University of Catania, Italy;IBAF Centre, CNR, Roccelletta di Borgia, Catanzaro, Italy;Department of Experimental Medicine and Biochemical Sciences, University of Rome "Tor Vergata", Rome, Italy;IRCCS "L. Spallanzani", Rome, Italy;MetaPhore Pharmaceuticals. Inc., 1910 Innerbelt Business Center Drive, St Louis, MO 63114, USA

关键词: FeTPMS;    AIDS dementia complex;    therapy;    astroglial cells;    Peroxynitrite;   

Others  :  1223250
DOI  :  10.1186/1471-2202-3-13

 received in 2002-05-21, accepted in 2002-09-16,  发布年份 2002

【 摘 要 】

Background

Oxidative stress has shown to contribute in the mechanisms underlying apoptotic cell death occuring in AIDS-dementia complex. Here we investigated the role of peroxynitrite in apoptosis occurring in astroglial cells incubated with supernatants of HIV-infected human primary macrophages (M/M).

Results

Flow cytometric analysis (FACS) of human cultured astrocytes shortly incubated with HIV-1-infected M/M supernatants showed apoptotic cell death, an effect accompanied by pronounced staining for nitrotyrosine (footprint of peroxynitrite) and by abnormal formation of malondialdehyde (MDA). Pretreatment of astrocytes with the peroxynitrite decomposition catalyst FeTMPS antagonized HIV-related astrocytic apoptosis, MDA formation and nitrotyrosine staining.

Conclusions

Taken together, our results suggest that inibition of peroxynitrite leads to protection against peroxidative stress accompanying HIV-related apoptosis of astrocytes. Overall results support the role of peroxynitrite in HIV-related programmed death of astrocytes and suggest the use of peroxynitrite decomposition catalyst to counteract HIV-1-related neurological disorders.

【 授权许可】

   
2002 Muscoli et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

【 图 表 】

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