R-spondins are involved in the ovarian differentiation in a teleost, medaka (Oryzias latipes)

BMC Developmental Biology

Yoshitaka Nagahama¹  Deshou Wang³  Sipra Mohapatra³  Gang Liu³  Limin Wu³  Xiangguo Yu³  Tapas Charkraborty²  Linyan Zhou⁴ 

[1] Institution for Collaborative Relations, Ehime University, 3 Bunkyo-cho, Matsuyama, 790-8577, Japan;Division of Molecular Environmental Endocrinology, National Institute for Basic Biology, Okazaki, 444-8585, Japan;Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), School of Life Science, Southwest University, Chongqing, 400715, P.R. China;Japan Society for the Promotion of Science (JSPS), 8 Ichiban-cho, Chiyoda-ku, Tokyo, 102-8472, Japan

关键词: Ovarian differentiation;    Expression profiles;    Molecular cloning;    Rspo1, 2, 3;   

Others  :  1086315
DOI  :  10.1186/1471-213X-12-36

 received in 2012-06-21, accepted in 2012-11-13,  发布年份 2012

【 摘 要 】

Background

In mammals, R-spondin (Rspo), an activator of the Wnt/β-catenin signaling pathway, has been shown to be involved in ovarian differentiation. However, the role of the Rspo/Wnt/β-catenin signaling pathway in fish gonads is still unknown.

Results

In the present study, full-length cDNAs of Rspo1, 2 and 3 were cloned from the gonads of medaka (Oryzias latipes). The deduced amino acid sequences of mRspo1-3 were shown to have a similar structural organization. Phylogenetic analysis showed that Rspo1, 2 and 3 were specifically clustered into three distinct clads. Tissue distribution revealed that three Rspo genes were abundantly expressed in the brain and ovary. Real-time PCR analysis around hatching (S33-5dah) demonstrated that three Rspo genes were specifically enhanced in female gonads from S38. In situ hybridization (ISH) analysis demonstrated that three Rspo genes were expressed in the germ cell in ovary, but not in testis. Fluorescence multi-color ISH showed that Rspo1 was expressed in both somatic cells and germ cells at 10dah. Exposure to ethinylestradiol (EE2) in XY individuals for one week dramatically enhanced the expression of three Rspo genes both at 0dah and in adulthood.

Conclusions

These results suggest that the Rspo-activating signaling pathway is involved in the ovarian differentiation and maintenance in medaka.

【 授权许可】

   
2012 Zhou et al.; licensee BioMed Central Ltd.

【 图 表 】

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