Induction of IFNα or IL-12 depends on differentiation of THP-1 cells in dengue infections without and with antibody enhancement

BMC Infectious Diseases

Kuender D Yang¹  Jiin-Tsuey Cheng²  Lin Wang³  Rong-Fu Chen¹ 

[1] Department of Medical Research and Development, Show Chwan Health Care System, Changhua, Taiwan;Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan;Department of Pediatrics, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan

关键词: IL-12;    IFNα;    CD123;    Differentiation;    Deneritic cells;    Monocyte;   

Others  :  1158740
DOI  :  10.1186/1471-2334-12-340

 received in 2012-05-15, accepted in 2012-12-04,  发布年份 2012

【 摘 要 】

Background

Appropriate induction of the early Th1 cytokine IL-12 is a critical defense directed against viral infection. We have previously shown that different viruses elicited either IL-12 or IFNα dependent Th1 reactions. Using dengue-2 virus, we sought to explore how dengue-2 induced IL-12 or IFNα expression by monocytic and its derived dendritic cells.

Methods

We employed human monocytic cell line, THP-1, to investigate whether differentiation of monocytic cells is involved in the switch between IFNα and IL-12 induction. Flow cytometry, RT-PCR and ELISA were respectively used to determine cell differentiation, IL-12 and IFNα mRNA expression and protein production.

Results

THP-1, expressing CD123, which is a plasmacytoid dendritic cell marker, but not CD14, CD11b or CD11c revealed IFNα mRNA expression while stimulated by dengue-2. In contrast, PMA-induced THP-1 differentiation toward monocytic cells expressed CD11b⁺, and CD14⁺, but not CD123, and revealed exclusively IL-12 expression while stimulated by dengue-2. Further studies showed that CD123⁺ expressing THP-1 cells elicited higher IFNα expression in dose and time dependent induction after infection, and PMA-induced monocytic differentiation of THP-1 cells revealed IL-12 expression. Antibody-dependent enhancement of DEN-2 infection significantly suppressed the DEN-2 induced IL-12 p40 expression in monocytic differentiated THP-1 cells.

Conclusions

Clarification and modulation of the early Th1 reaction in different monocytic cells may change or prevent complication from dengue infection.

【 授权许可】

   
2012 Chen et al.; licensee BioMed Central Ltd.

【 图 表 】

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