Cost effective improvement in the protocol for detection of haemoglobin variants –a step forward in quality assurance

BMC Research Notes

Mohammad Khurshid¹  Natasha Ali¹ 

[1] Department of Pathology and Microbiology/Oncology, The Aga Khan University and Hospital, Stadium Road, P.O Box 3500, Karachi, 74800, Pakistan

关键词: Haemoglobinopathy;    Peripheral blood film;    HPLC;   

Others  :  1142703
DOI  :  10.1186/1756-0500-6-192

 received in 2012-12-19, accepted in 2013-05-08,  发布年份 2013

【 摘 要 】

Background

We report the results of a cost effective improvement in the protocol for detection of haemoglobin variants which incorporates the findings of peripheral blood film along with the results of HPLC.

Findings

A total of n = 10,844 samples were received from January 2011 till August 2011. Diagnosis of haemoglobinopathy was made in n = 1123 samples while other abnormalities included iron deficiency anaemia, megaloblastic anaemia, malarial parasite, autoimmune haemolytic anaemia and G6PD deficiency (n = 2473).

Conclusion

We diagnosed 23% of abnormalities other than haemoglobinopathy by reviewing peripheral smear of samples received for detection of haemoglobin variants. This resulted in providing proper diagnosis to the referring physician without increment in cost.

【 授权许可】

   
2013 Ali and khurshid; licensee BioMed Central Ltd.

【 图 表 】

Figure 5.

【 参考文献 】

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