First-generation linkage map for the European tree frog (Hyla arborea) with utility in congeneric species

BMC Research Notes

Nicolas Perrin¹  Alan Brelsford¹  Christophe Dufresnes¹ 

[1] Department of Ecology and Evolution, Biophore building, University of Lausanne, 1015 Lausanne, Switzerland

关键词: Transcriptome;    Recombination;    Population genetics;    Microsatellites;    Hylid frogs;    Heterochiasmy;    Conservation;   

Others  :  1118168
DOI  :  10.1186/1756-0500-7-850

 received in 2014-07-28, accepted in 2014-11-12,  发布年份 2014

【 摘 要 】

Background

Western Palearctic tree frogs (Hyla arborea group) represent a strong potential for evolutionary and conservation genetic research, so far underexploited due to limited molecular resources. New microsatellite markers have recently been developed for Hyla arborea, with high cross-species utility across the entire circum-Mediterranean radiation. Here we conduct sibship analyses to map available markers for use in future population genetic applications.

Findings

We characterized eight linkage groups, including one sex-linked, all showing drastically reduced recombination in males compared to females, as previously documented in this species. Mapping of the new 15 markers to the ~200 My diverged Xenopus tropicalis genome suggests a generally conserved synteny with only one confirmed major chromosome rearrangement.

Conclusions

The new microsatellites are representative of several chromosomes of H. arborea that are likely to be conserved across closely-related species. Our linkage map provides an important resource for genetic research in European Hylids, notably for studies of speciation, genome evolution and conservation.

【 授权许可】

   
2014 Dufresnes et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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