Isolation and characterization of 11 novel microsatellite loci in a West African leaf-nosed bat, Hipposideros aff. ruber

BMC Research Notes

Adam J Stow⁵  Marco Tschapka⁴  Christian Drosten²  Michael G Gardner¹  Peter Vallo³  Heather J Baldwin⁵ 

[1] Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, South Australia 5000, Australia;Institute of Virology, University of Bonn Medical Center, Sigmund-Freud-Str 25, 53105 Bonn, Germany;Institute of Vertebrate Biology, v. v. i., Academy of Sciences of the Czech Republic, Květná 8, 603 65 Brno, Czech Republic;Smithsonian Tropical Research Institute, PO Box 0843–03092, Balboa, Panama;Department of Biological Sciences, Macquarie University, Sydney, New South Wales 2109, Australia

关键词: Bat;    Hipposideridae;    Microchiroptera;    Population genetics;    Microsatellites;    Hipposideros caffer;    Hipposideros ruber;   

Others  :  1129823
DOI  :  10.1186/1756-0500-7-607

 received in 2014-07-08, accepted in 2014-08-28,  发布年份 2014

【 摘 要 】

Background

Noack’s leaf-nosed bat, Hipposideros ruber, is a cryptic species within the Hipposideros caffer species complex. Despite a widespread distribution in Africa and being host to potentially zoonotic viruses, the genetic structure and ecology of H. ruber is poorly known. Here we describe the development of 11 novel polymorphic microsatellite loci to facilitate the investigation of genetic structure.

Findings

We selected 20 microsatellite sequences identified from high throughput sequence reads and PCR amplified these for 38 individuals, yielding 11 consistently amplifying and scorable loci. The number of alleles per locus ranged from two to 12, and observed heterozygosities from 0.00 to 0.865. No evidence of linkage disequilibrium was observed, and nine of the markers showed no departure from Hardy-Weinberg equilibrium. We demonstrate successful amplification in two closely related species and two divergent lineages of the H. caffer species complex.

Conclusions

These new markers will provide a valuable tool to investigate genetic structure in the poorly understood Hipposideros caffer species complex.

【 授权许可】

   
2014 Baldwin et al.; licensee BioMed Central Ltd.

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