Allyl isothiocyanate induced stress response in Caenorhabditis elegans

BMC Research Notes

Martin JT Reaney³  Youn Young Shim³  Robert T Tyler¹  AkalRachna K Saini² 

[1] 51 Campus Drive, Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5A8, Canada;343-111 Research Drive, Helix BioPharma Corp, Saskatoon, Saskatchewan S7N 3R2, Canada;51 Campus Drive, Department of Plant Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5A8, Canada

关键词: ELISA;    toxicity;    HSP70;    glucosinolate;    myrosinase;    Brassica;   

Others  :  1166938
DOI  :  10.1186/1756-0500-4-502

 received in 2010-12-05, accepted in 2011-11-17,  发布年份 2011

【 摘 要 】

Background

Allyl isothiocyanate (AITC) from mustard is cytotoxic; however the mechanism of its toxicity is unknown. We examined the effects of AITC on heat shock protein (HSP) 70 expression in Caenorhabditis elegans. We also examined factors affecting the production of AITC from its precursor, sinigrin, a glucosinolate, in ground Brassica juncea cv. Vulcan seed as mustard has some potential as a biopesticide.

Findings

An assay to determine the concentration of AITC in ground mustard seed was improved to allow the measurement of AITC release in the first minutes after exposure of ground mustard seed to water. Using this assay, we determined that temperatures above 67°C decreased sinigrin conversion to AITC in hydrated ground B. juncea seed. A pH near 6.0 was found to be necessary for AITC release. RT-qPCR revealed no significant change in HSP70A mRNA expression at low concentrations of AITC (< 0.1 μM). However, treatment with higher concentrations (> 1.0 μM) resulted in a four- to five-fold increase in expression. A HSP70 ELISA showed that AITC toxicity in C. elegans was ameliorated by the presence of ground seed from low sinigrin B. juncea cv. Arrid.

Conclusions

• AITC induced toxicity in C. elegans, as measured by HSP70 expression.

• Conditions required for the conversion of sinigrin to AITC in ground B. juncea seed were determined.

• The use of C. elegans as a bioassay to test AITC or mustard biopesticide efficacy is discussed.

【 授权许可】

   
2011 Reaney et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Bones AM, Rossiter JT: The myrosinase-glucosinolate system, its organization and biochemistry. Physiol Plant 1996, 97:194-208.
• [2]Fahey JW, Zalcmann AT, Talalay P: The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochem 2001, 56:5-51.
• [3]Fenwick GR, Heaney RK: Glucosinolates and their breakdown products in cruciferous crops, foods and feeding stuffs. Food Chem 1983, 11:249-271.
• [4]Mithen R: Glucosinolates-biochemistry, genetics and biological activity. Plant Growth Reg 2001, 34:91-103.
• [5]Noble RRP, Harvey SG, Sams CE: Toxicity of Indian mustard and allyl isothiocyanate to masked chafer beetle larvae. Plant Health Prog 2002.
• [6]Smolinska U, Morra MJ, Knudsen GR, James RL: Isothiocyanates produced by Brassicaceae species as inhibitors of Fusarium oxysporum. Plant Dis 2003, 87:407-412.
• [7]Tsao R, Peterson CJ, Coats JR: Glucosinolate breakdown products as insect fumigants and their effect on carbon dioxide emission of insects. BMC Ecology 2002, 2:5. BioMed Central Full Text
• [8]Mayton HS, Olivier C, Vaughn SF, Loria R: Correlation of fungicidal activity of Brassica species with allyl isothiocyanate production in macerated leaf tissues. Phytopath 1996, 86:267-271.
• [9]Winther M, Nielsen PV: Active packaging of cheese with allyl isothiocynate, an alternative to modified atmosphere packaging. J Food Protect 2006, 69:2430-2435.
• [10]Tunc SE, Chollet E, Chalier P, Preziosi-Belloy L, Gontard N: Combined effect of volatile antimicrobial agents on the growth of Penicillium notatum. Int J Food Microbiol 2007, 113:263-270.
• [11]Potter MJ, Davies K, Rathjen AJ: Suppressive impact of glucosinolates in Brassica vegetative tissues on root lesion nematode Pratylenchus neglectus. J Chem Ecol 1997, 24:67-80.
• [12]Buskov S, Serra B, Rosa E, Sorenses H, Sorensen JC: Effects of intact glucosinolates and products produced from glucosinolates in myrosinase-catalyzed hydrolysis on the potato cyst nematode (Globodera rostochiensis Cv. Woll). J Agric Food Chem 2002, 50:690-695.
• [13]Vierling E: The roles of heat shock proteins in plants. Ann Rev Plant Physiol Plant Mol Biol 1991, 42:579-620.
• [14]Parsell DA, Lindquist AS: Heat shock proteins and stress responses. In The Biology of heat shock proteins and molecular chaperones. Edited by Morimoto RI, Tissieres A, Georgopoulos C. New York: Cold Spring Harbor Laboratory Press; 1994:457-494.
• [15]Suri SS, Dhindsa RS: A heat-activated MAP kinase (HAMK) as a mediator of heat shock response in tobacco cells. Plant Cell Environ 2008, 31:218-226.
• [16]Heschl MF, Baillie DL: Characterization of the hsp70 multigene family of Caenorhabditis elegans. DNA 1998, 8:233-243.
• [17]Raquet D: Estimation of essential oil in mustard. Ann Chim Anal 1912, 17:174-178.
• [18]Kirk LD, Mustakas GC, Griffin EL, Booth AN: Crambe seed processing: decomposition of glucosinolates (thioglucosides) with chemical additives. J Am Oil Chem Soc 1971, 48:845-850.
• [19]Eylen DV, Hendrickx IM, Loey AV: Temperature and pressure stability of mustard seed (Sinapis alba L.) myrosinase. Food Chem 2006, 97:263-271.
• [20]Verker R, Dekker M: Glucosinolates and myrosinase activity in red cabbage (Brassica oleracea L. Var. Capitita f. rubra DC.) after various microwave treatments. J Agric Food Chem 2004, 52:7318-7323.