Physalis angulata induces in vitro differentiation of murine bone marrow cells into macrophages

BMC Cell Biology

Edilene O Silva³  Jose Luiz M Do Nascimento¹  Amanda Anastácia P Hage³  Luis Henrique S Farias³  Ana Paula D Rodrigues²  Bruno José Martins da Silva³ 

[1] Instituto de Ciências Biológicas, Laboratório de Neuroquímica Molecular e Celular, Universidade Federal do Pará, Avenida Augusto Corrêa, 01, Bairro Guamá, 660975-110 Belém, Pará, Brazil;Laboratório de Microscopia Eletrônica, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde do Ministério da Saúde, Belém, Pará, Brazil;Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagem, Rio de Janeiro, Brazil

关键词: Physalis angulata;    Bone marrow cells;    Cell differentiation;   

Others  :  1230195
DOI  :  10.1186/1471-2121-15-37

 received in 2014-05-22, accepted in 2014-09-24,  发布年份 2014

【 摘 要 】

Background

The bone marrow is a hematopoietic tissue that, in the presence of cytokines and growth factors, generates all of the circulating blood cells. These cells are important for protecting the organism against pathogens and for establishing an effective immune response. Previous studies have shown immunomodulatory effects of different products isolated from plant extracts. This study aimed to evaluate the immunomodulatory properties of aqueous Physalis angulata (AEPa) extract on the differentiation of bone marrow cells.

Results

Increased cellular area, higher spreading ability and several cytoplasmatic projections were observed in the treated cells, using optical microscopy, suggesting cell differentiation. Furthermore, AEPa did not promote the proliferation of lymphocytes and polymorphonuclear leukocytes, however promotes increased the number of macrophages in the culture. The ultrastructural analysis by Transmission Electron Microscopy of treated cells showed spreading ability, high number of cytoplasmatic projections and increase of autophagic vacuoles. Moreover, a high level of LC3b expression by treated cells was detected by flow cytometry, suggesting an autophagic process. Cell surface expression of F4/80 and CD11b also indicated that AEPa may stimulate differentiation of bone marrow cells mainly into macrophages. In addition, AEPa did not differentiate cells into dendritic cells, as assessed by CD11c analysis. Furthermore, no cytotoxic effects were observed in the cells treated with AEPa.

Conclusion

Results demonstrate that AEPa promotes the differentiation of bone marrow cells, particularly into macrophages and may hold promise as an immunomodulating agent.

【 授权许可】

   
2014 da Silva et al.; licensee BioMed Central Ltd.

【 图 表 】

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