Antibiotic resistance, virulence determinants and production of biogenic amines among enterococci from ovine, feline, canine, porcine and human milk

BMC Microbiology

Juan M Rodríguez²  Carmen Torres¹  Miguel A Álvarez³  María Fernández³  Leonides Fernández²  Virginia Martín²  María López¹  Antonio Maldonado-Barragán⁵  Irene Chico⁴  Victor Ladero³  Esther Jiménez⁴ 

[1] Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain;Probisearch. c/Santiago Grisolía, Tres Cantos 2. 28760, Spain;Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Paseo Río Linares s/n 33300, Villaviciosa, Spain;Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, Ciudad Universitaria, Avda. Puerta de Hierro, Madrid s/n. 28040, Spain;Departamento de Biotecnología de Alimentos, Instituto de la Grasa-CSIC, Sevilla 41012, Spain

关键词: Biogenic amines;    Antibiotic resistance;    Virulence;    Mammals;    Milk;    Enterococcus;   

Others  :  1142410
DOI  :  10.1186/1471-2180-13-288

 received in 2013-07-04, accepted in 2013-12-04,  发布年份 2013

【 摘 要 】

Background

Recent studies have shown that mammalian milk represents a continuous supply of commensal bacteria, including enterococci. The objectives of this study were to evaluate the presence of enterococci in milk of different species and to screen them for several genetic and phenotypic traits of clinical significance among enterococci.

Results

Samples were obtained from, at least, nine porcine, canine, ovine, feline and human healthy hosts. Enterococci could be isolated, at a concentration of 1.00 × 10² -1.16 × 10³ CFU/ml, from all the porcine samples and, also from 85, 50, 25 and 25% of the human, canine, feline and ovine ones, respectively. They were identified as Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Enterococcus casseliflavus and Enterococcus durans. Among the 120 initial enterococcal isolates, 36 were selected on the basis of their different PFGE profiles and further characterized. MLST analysis revealed a wide diversity of STs among the E. faecalis and E. faecium strains, including some frequently associated to hospital infections and novel STs. All the E. faecalis strains possessed some of the potential virulence determinants (cad, ccf, cob, cpd, efaA_fs, agg2, gelE, cylA, esp_fs) assayed while the E. faecium ones only harboured the efaA_fm gene. All the tested strains were susceptible to tigecycline, linezolid and vancomycin, and produced tyramine. Their susceptibility to the rest of the antimicrobials and their ability to produce other biogenic amines varied depending on the strain. Enterococci strains isolated from porcine samples showed the widest spectrum of antibiotic resistance.

Conclusions

Enterococci isolated from milk of different mammals showed a great genetic diversity. The wide distribution of virulence genes and/or antibiotic resistance among the E. faecalis and E. faecium isolates indicates that they can constitute a reservoir of such traits and a risk to animal and human health.

【 授权许可】

   
2013 Jiménez et al.; licensee BioMed Central Ltd.

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