Effects of rhodiola crenulata on mice hearts under severe sleep apnea

BMC Complementary and Alternative Medicine

Shin-Da Lee⁷  Chih-Yang Huang³  Yi-fan Liu⁷  Shiu-Min Cheng⁵  Yu-Lan Yeh⁴  Yueh-Min Lin⁴  Mei-Hsin Lai⁶  Pei-Ying Pai¹  Jaung-Geng Lin²  Mei-Chih Lai² 

[1] Graduate Institute of Clinical Medical Science, China Medical University and Hospital, Taichung, Taiwan;Graduate Institute of Chinese Medicine, China Medical University, Taichung, Taiwan;Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan;Department of Medical Technology, Jen-The Junior College of Medicine, Nursing and Management, Miaoli, Taiwan;Department of Psychology, Asia University, Taichung, Taiwan;Department of Nursing, Master Program, Hungkuang University, Taichung, Taiwan;Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, No. 91, Hsueh-Shih Road, Taichung, Taiwan

关键词: VEGF;    Survival;    Rhodiola;    Hypoxia;    Cardiac;    Caspase;    Apoptosis;   

Others  :  1217041
DOI  :  10.1186/s12906-015-0698-0

 received in 2015-02-03, accepted in 2015-05-28,  发布年份 2015

【 摘 要 】

Background

The goal of this study is to determine if Rhodiola Crenulata (RC) has protective effects on mice hearts with severe sleep apnea model.

Methods

Sixty-four C57BL/6 J mice 5–6 months old were distributed into 4 groups i.e. Control group (21 % O ₂ , 24 h per day, 8 weeks, n = 16); Hypoxia group (Hypoxia: 7 % O ₂60 s, 20 % O ₂alternating 60 s, 8 h per day, 8 weeks, n = 16); Hypoxia + 90RC and Hypoxia + 270RC group (Hypoxia for 1st 4 weeks and hypoxia pretreated 90 mg/Kg and 270 mg/Kg Rhodiola Crenulata by oral gavage per day for 2nd 4 weeks, each n = 16). Excised hearts from 4 groups of mice were analyzed for heart weight index changes using H&E staining, TUNEL-positive assays and Western Blotting protein.

Results

Cardiac widely dispersed TUNEL-positive apoptotic cells in mice hearts were less in Hypoxia + RC90 and Hypoxia + RC270 than those in Hypoxia. Compared with Hypoxia, the protein levels of Fas ligand, Fas death receptors, Fas-Associated Death Domain (FADD), activated caspase 8, and activated caspase 3 (Fas dependent apoptotic pathways) were decreased in Hypoxia + RC90, Hypoxia + RC270. The protein levels of Bad, Bax, t-Bid, activated caspase 9, activated caspase 3 (mitochondria dependent apoptotic pathway) were less in Hypoxia + RC90, Hypoxia + RC270 than those in hypoxia. The protein levels of Bcl2, Bcl-xL, p-Bad (Bcl2-realted anti-apoptotic pathway) and VEGF, p-PI3k, p-AKT (VEGF-related pro-survival pathway) were higher in Hypoxia + RC90, Hypoxia + RC270 than those in hypoxia.

Conclusions

Our findings suggest that Rhodiola Crenulata have protective effects on chronic intermittent hypoxia-induced cardiac widely dispersed apoptosis via Fas-dependent and mitochondria-dependent apoptotic and VEGF-related pro-survival pathway.

【 授权许可】

   
2015 Lai et al.

【 图 表 】

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