Proposal for a method to estimate nutrient shock effects in bacteria

BMC Research Notes

Maria J Vieira¹  Charles W Keevil²  Carina Almeida¹  Laura Cerqueira¹  Lúcia C Simões¹  Sofia M Bragança¹  Nuno F Azevedo¹ 

[1] IBB-Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal;Environmental Healthcare Unit, Microbiology Group, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, SO16 7PX, United Kingdom

关键词: Substrate-accelerated death;    Nutrient stress;    Osmotic shock;    Nutrient shock;   

Others  :  1165973
DOI  :  10.1186/1756-0500-5-422

 received in 2012-02-07, accepted in 2012-07-12,  发布年份 2012

【 摘 要 】

Background

Plating methods are still the golden standard in microbiology; however, some studies have shown that these techniques can underestimate the microbial concentrations and diversity. A nutrient shock is one of the mechanisms proposed to explain this phenomenon. In this study, a tentative method to assess nutrient shock effects was tested.

Findings

To estimate the extent of nutrient shock effects, two strains isolated from tap water (Sphingomonas capsulata and Methylobacterium sp.) and two culture collection strains (E. coli CECT 434 and Pseudomonas fluorescens ATCC 13525) were exposed both to low and high nutrient conditions for different times and then placed in low nutrient medium (R2A) and rich nutrient medium (TSA).

The average improvement (A.I.) of recovery between R2A and TSA for the different times was calculated to more simply assess the difference obtained in culturability between each medium. As expected, A.I. was higher when cells were plated after the exposition to water than when they were recovered from high-nutrient medium showing the existence of a nutrient shock for the diverse bacteria used. S. capsulata was the species most affected by this phenomenon.

Conclusions

This work provides a method to consistently determine the extent of nutrient shock effects on different microorganisms and hence quantify the ability of each species to deal with sudden increases in substrate concentration.

【 授权许可】

   
2012 Azevedo et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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