Evaluation of the wound healing property of Commiphora guidottii Chiov. ex. Guid.

BMC Complementary and Alternative Medicine

Franz Bucar¹  Peter Lindemann²  Avijit Mazumder³  Daniel Bisrat⁴  Kaleab Asres⁴  Michael Gebrehiwot⁴ 

[1]Department of Pharmacognosy, Institute of Pharmaceutical Sciences, Karl-Franzens University Graz, Universitaetsplatz 4/1, Graz, A-8010, Austria

[2]Divison Pharmaceutical Biology and Pharmacology, Institute of Pharmacy, Martin-Luther-University, Halle-Wittenberg, Hoher Weg 8, Halle, D-06120, Germany

[3]Department of Pharmaceutical Technology, Noida Institute of Engineering and Technology, 19 Knowledge Park II, Institutional Area, Greater Noida 201306, India

[4]Department of Pharmaceutical Chemistry and Pharmacognosy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia

关键词: Antimicrobial;    Anti-inflammatory;    Wound healing;    Resin;    Essential oil;    Commiphora guidottii;   

Others  :  1222533
DOI  :  10.1186/s12906-015-0813-2

 received in 2014-12-03, accepted in 2015-08-10,  发布年份 2015

【 摘 要 】

Background

The traditional use of the oleo-gum-resin of Commiphora guidottii Chiov. ex. Guid., which is commonly called scented myrrh, for topical treatment of wound is well documented. The major objective of the present study was to investigate the essential oil and resin obtained from C. guidottii for their potential wound healing properties. Due to their influence on the wound healing process, the anti-inflammatory and antimicrobial activities of scented myrrh have also been investigated.

Methods

Powdered oleo-gum-resin of C. guidottii was steam-distilled to obtain essential oil, and the resin was extracted from the marc with MeOH and filtered. The TLC fingerprint profile of the resin has been recorded by using silica gel GF ₂₅₄as stationary phase. The essential oil components were identified and quantified by GC-MS. Ointments prepared from the essential oil (4 % v/w) and the resin (5 % w/w) were used for wound healing activity tests. Toxicity of the formulated ointments was investigated according to Draize skin irritation test. Acute anti-inflammatory effect in mice was evaluated using carrageenan induced mouse hind paw oedema model. Antimicrobial activity tests were carried out using disk diffusion and broth dilution techniques against 21 pathogenic bacterial and 4 fungal strains.

Results

Ointment formulations of both the oil and resin were found to be non-irritant at the concentrations used and showed significant (p < 0.05-0.001) increase in wound contraction rate, shorter epithelization time and higher skin breaking strength as compared to the negative control. Overall, the antibacterial and antifungal activities of the oil and resin were comparable with the standard antibiotics ciprofloxacin and griseofulvin, respectively.

Conclusion

The results confirm that scented myrrh possesses genuine wound healing activity supporting the traditional use of the plant.

【 授权许可】

   
2015 Gebrehiwot et al.

【 图 表 】

Fig. 1.

Fig. 2.

【 参考文献 】

• [1]Martin P. Wound healing aiming for perfect skin regeneration. Science. 1997; 276:75-81.
• [2]Robson MC, Steed DL, Franz MG. Wound healing: biologic features and approaches to maximize healing trajectories. Curr Probl Surgery. 2001; 38:72-140.
• [3]Prasad V, Dorle K. Evaluation of ghee based formulation for wound healing activity. J Ethnopharmacol. 2006; 107:38-47.
• [4]Omale J, Issac AV. Excision and incision wound healing potential of Saba florida (Benth) leaf extract in Rattus novergicus.Int J Pharm Biomed Res. 2010;1:101–7.
• [5]Pirbalouti AG, Koohpayeh A, Karimi I. The wound healing activity of flower extracts of Punica granatum and Achillea kellalensis in Wistar rats. Acta Pol Pharm. 2010; 67:107-110.
• [6]Thulin M, Claeson P. The botanical origin of scented myrrh (Bissabol or Habak Hadi). Econ Bot. 1991; 45:487-494.
• [7]Claeson P, Samuelsson G. Screening of some Somalian medicinal plants for antidiarrhoeal effects in mice. Phytother Res. 1989; 3:180-183.
• [8]Shen T, Li G-H, Wang X-N, Lou H-X. The genus Commiphora: review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol. 2012; 142:319-330.
• [9]Hanuš LO, Řezanka T, Dembitsky V, Moussaieff A. Myrrh – Commiphora chemistry. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2005; 149:3-28.
• [10]Zygmunt P, Claeson P. Inhibitory effects of the sesquiterpene T-cadinol on contractile responses in the isolated guinea-pig ileum. Phytother Res. 1991; 5:142-144.
• [11]Claeson P, Rådström P, Sköld O, Nilsson Å, Höglund S. Bactericidal effect of the sesquiterpene T-cadinol on Staphylococcus aureus. Phytother Res. 1992; 6:94-98.
• [12]Andersson M, Bergendorff O, Shan R, Zygmunt P, Sterner O. Minor components with smooth muscle relaxing properties from scented myrrh (Commiphora guidottii). Planta Med. 1997; 63:251-254.
• [13]Guide for the Care and Use of Laboratory Animals. National Academy Press, Washington D.C.; 1996.
• [14]Trease GE, Evans WC. A Text Book of Pharmacognosy. 3rd ed. Bailliére Tindall, London; 1989.
• [15]Adams RP. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. 4th ed. Allured Publ Corp, Illinois; 2007.
• [16]Jordán MJ, Margaría CA, Shaw PE, Goodner KL. Volatile components and aroma active compounds in aqueous essence and fresh pink guava fruit puree (Psidium guajava L.) by GC-MS and multidimensional GC/GC-O. J Agric Food Chem. 2003; 51:1421-1426.
• [17]Hazzit M, Baaliouamer A, Faleiro ML, Miguel MG. Composition of the essential oils of Thymus and Origanum species from Algeria and their antioxidant and antimicrobial activities. J Agric Food Chem. 2006; 54:6314-6321.
• [18]Oliveira AL, Lopes RB, Cabral FA, Eberlin MN. Volatile compounds from pitanga fruit (Eugenia uniflora L.). Food Chem. 2006; 99:1-5.
• [19]Jones P, Kinghorn D. Extraction of plant secondary metabolites. Methods in Biothechnology of Natural Products Isolation. 2nd ed. Sarker D, Latif Z, Gray A, editors. Humana Press, New Jersey; 2006.
• [20]Sasidharan S, Nilawatyi R, Xavier R, Latha L, Amala R. Wound healing potential of Elaeis guineensis Jacq leaves in an infected albino rat model. Molecules. 2010; 15:3186-3199.
• [21]Gatsing D, Aliyu R, Kuiate R, Garba H, Jaryum H, Tedongmo N, et al. Toxicological evaluation of the aqueous extract of Allium sativum bulbs on laboratory mice and rats. Cameroon J Exp Biol. 2005;1:39–45.
• [22]Nair SS, Mathew M, Sreena K. Evaluation of skin irritation of herbal antioxidant cream. Asian J Biochem Pharm Res. 2012; 2:184-189.
• [23]Draize J. Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics. Association Food Drug Officials USA. 1959; 49:2-56.
• [24]British Pharmacopoeia. Department of Health and Social Security Scottish Home and Health Department, Office of the British Pharmacopoeia Commission, UK. 1988, 2: 713.
• [25]Barua CC, Talukdar A, Begum SA, Sarma DK, Fathalk DC, Barua AG, et al. Wound healing activity of methanolic extract of leaves of Alternanthrea brasiliana Kuntz using in vivo and in vitro model. Indian J Exp Biol. 2009;47:1001–5.
• [26]Agarwal PK, Singh A, Gaurav K, Goel S, Khanna HD, Goel RK. Evaluation of wound healing activity of extracts of plantain banana (Musa sapientum var. paradisiaca) in rats. Indian J Exp Biol. 2009; 47:32-40.
• [27]Annan K, Dickson R. Evaluation of wound healing actions of Hoslundia opposite Vahl, Anthocleista nobilis G. Don. and Balanites aegyptiaca L. J Sci Technol. 2008; 28:26-35.
• [28]Kokane DD, More RY, Kale MB, Nehete MN, Mehendale PC, Gadgoli CH. Evaluation of wound healing activity of root of Mimosa pudica. J Ethnopharmacol. 2009; 124:311-315.
• [29]Akkol EK, Süntar I, Orhan IE, Keles H, Kan A, Çoksari G. Assessment of dermal wound healing and in vitro antioxidant properties of Avena sativa L. J Cereal Sci. 2011; 53:285-290.
• [30]Leach AA. Notes on a modification of the Neuman and Logan method for the determination of the hydroxyproline. Biochem J. 1960; 74:70-71.
• [31]Gurung S, Skalko-Basnet N. Wound healing properties of Carica papaya latex: in vivo evaluation in mice burn model. J Ethnopharmacol. 2009; 121:338-341.
• [32]Padilha MM, Vilela FC, Rocha CQ, Dias MJ, Soncini R, dos Santos MH, et al. Anti-inflammatory properties of Morus nigra leaves. Phytother Res. 2010;24:1496–500.
• [33]Marrassini C, Acevedo C, Miño J, Ferraro G, Gorzalczany S. Evaluation of antinociceptive, anti-inflammatory activities and phytochemical analysis of aerial parts of Urtica urens L. Phytother Res. 2010; 24:1807-1812.
• [34]Mitchell J, Carter W. Modeling antimicrobial activity of CloroxR using an agar-diffusion test: a new twist on an old experiment. Bioscene. 2000; 26:9-13.
• [35]Clinical and Laboratory Standards Institute. Method for Antimicrobial Susceptibility Testing of Aanaerobic Bacteria. Approved standard, 7th edition. 2006, 26:2.
• [36]EPA (US) Dermal Classification System. Federal Insecticide, Fungicide, Rodenticide Act, Pesticide Assessment Guidelines, Subdivision F: Hazard evaluation: humans and domestic animals. Addendum 3 on data reporting; 1988.
• [37]Mekonnen Y, Dekebo A, Dagne. Toxicity study in mice of resins of three Commiphora species. SINET: Ethiop J Sci. 2003; 26:151-153.
• [38]Craveiro A, Corsano S, Proietti G, Strappaghetti G. Constituents of essential oil of Commiphora guidottii. Planta Med. 1983; 48:97-98.
• [39]Ejaz S, Chekarova I, Cho JW, Lee SY, Ashraf S, Lim SW. Effect of aged garlic extract on wound healing: a new frontier in wound management. Drug Chem Toxicol. 2009; 32:191-203.
• [40]Getie M, Gebre-Mariam T, Rietz R, Höhne C, Huschaka C, Schmditke M, et al. Evaluation of the antimicrobial and anti-inflammatory activities of the medicinal plants Dodonaea viscosa, Rumex nervosus and Rumex abyssinicus. Fitoterapia. 2003;74:139–43.
• [41]Wang JP, Ruan JL, Cai YL, Luo Q, Xu HX, Wu YX. In vitro and in vivo evaluation of the wound healing properties of Siegesbeckia pubescens. J Ethnopharmacol. 2011; 134:1033-1038.
• [42]Nayak S, Nalabothu P, Sandiford S, Bhogadi V, Adogwa A. Evaluation of wound healing activity of Allamanda cathartica L. and Laurus nobilis L. extracts on rats. BMC Complem Altern Med. 2006;6:12.
• [43]Taşkiran D, Taşkiran E, Yercan H, Kutay FZ. Quantification of total collagen in rabbit tendon by the Sirius Red method. Turkish J Med Sci. 1999; 29:7-9.
• [44]Iyyam Pillai S, Palsamy P, Subramanian S, Kandaswamy M. Wound healing properties of Indian propolis studied on excision wound-induced rats. Pharm Biol. 2010; 48:1198-2106.
• [45]Di Rosa M, Giroud JP, Willoughby DA. Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. J Pathol. 1971; 104:15-29.
• [46]Patrone JB, Stein DC. Effect of gonococcal lipooligosaccharide variation on human monocytic cytokine profile. BMC Microbiol. 2007; 7:7. BioMed Central Full Text
• [47]Raina R, Prawez S, Verma PK, Pankaj NK. Medicinal plants and their role in wound healing. Vet Scan. 2008; 3:2.
• [48]Gupta AK, Cooper EA. Update in antifungal therapy of dermatophytosis. Mycopathologia. 2008; 166:353-367.
• [49]Pradhan D, Panda P, Tripathy G. Wound healing activity of aqueous and methanolic bark extracts of Vernonia arborea Buch.-Ham. in wistar rats. Nat Prod Rad. 2009; 8:6-11.
• [50]Deshmukh PT, Fernandes J, Atul A, Toppoa E. Wound healing activity of Calotropis gigantea root bark in rats. J Ethnopharmacol. 2009; 125:178-181.
• [51]Biswas TK, Auddy B, Bhattacharya NP, Bhattacharya S, Mukherjee B. Wound healing activity of human placental extracts in rats. Acta Pharmacol Sin. 2001; 22:1113-1116.
• [52]Houghton PJ, Hylands PJ, Mensah AY, Hensel A, Deters AM. In vitro tests and ethnopharmacological investigations: wound healing as an example. J Ethnopharmacol. 2005; 100:100-107.