Comparison of PCR/Electron spray Ionization-Time-of-Flight-Mass Spectrometry versus Traditional Clinical Microbiology for active surveillance of organisms contaminating high-use surfaces in a burn intensive care unit, an orthopedic ward and healthcare workers

BMC Infectious Diseases

Clinton K Murray²  Katrin Mende¹  J William Costerton⁶  Tracy L Spirk⁶  Joseph R Hsu⁴  Joseph C Wenke⁴  Kevin K Chung⁴  Helen K Crouch⁵  Charles H Guymon⁴  Garth D Ehrlich³  Mayra A Castillo⁵  Rachael E Kreft⁶  Heather C Yun² 

[1] Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD, USA;Uniformed Services University, Bethesda, MD, USA;Departments of Microbiology and Immunology, and Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Pittsburgh, PA, USA;US Army Institute of Surgical Research, San Antonio, TX, USA;San Antonio Military Medical Center, San Antonio, TX, USA;Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA, USA

关键词: Environment;    Contamination;    Microbiology;    Ibis;    PCR/ESI-TOF-MS;   

Others  :  1159639
DOI  :  10.1186/1471-2334-12-252

 received in 2012-07-11, accepted in 2012-09-20,  发布年份 2012

【 摘 要 】

Background

Understanding nosocomial pathogen transmission is restricted by culture limitations. Novel platforms, such as PCR-based electron spray ionization-time-of-flight-mass spectrometry (ESI-TOF-MS), may be useful as investigational tools.

Methods

Traditional clinical microbiology (TCM) and PCR/ESI-TOF-MS were used to recover and detect microorganisms from the hands and personal protective equipment of 10 burn intensive care unit (ICU) healthcare workers providing clinical care at a tertiary care military referral hospital. High-use environmental surfaces were assessed in 9 burn ICU and 10 orthopedic patient rooms. Clinical cultures during the study period were reviewed for pathogen comparison with investigational molecular diagnostic methods.

Results

From 158 samples, 142 organisms were identified by TCM and 718 by PCR/ESI-TOF-MS. The molecular diagnostic method detected more organisms (4.5 ± 2.1 vs. 0.9 ± 0.8, p < 0.01) from 99% vs. 67% of samples (p < 0.01). TCM detected S. aureus in 13 samples vs. 21 by PCR/ESI-TOF-MS. Gram-negative organisms were less commonly identified than gram-positive by both methods; especially by TCM. Among all detected bacterial species, similar percentages were typical nosocomial pathogens (18-19%) for TCM vs. PCR/ESI-TOF-MS. PCR/ESI-TOF-MS also detected mecA in 112 samples, vanA in 13, and KPC-3 in 2. MecA was associated (p < 0.01) with codetection of coagulase negative staphylococci but not S. aureus. No vanA was codetected with enterococci; one KPC-3 was detected without Klebsiella spp.

Conclusions

In this pilot study, PCR/ESI-TOF-MS detected more organisms, especially gram-negatives, compared to TCM, but the current assay format is limited by the number of antibiotic resistance determinants it covers. Further large-scale assessments of PCR/ESI-TOF-MS for hospital surveillance are warranted.

【 授权许可】

   
2012 Yun et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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