Claudins play a role in normal and tumor cell motility

BMC Cell Biology

Heidi K Baumgartner¹  Monique A Spillman¹  Patricia G Webb¹ 

[1] Department of Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, Reproductive Sciences Mail Stop 8613, Aurora, CO 80045, USA

关键词: Ovarian cancer;    Breast cancer;    Mammary cells;    Collagen;    Motility;    Claudin;   

Others  :  855382
DOI  :  10.1186/1471-2121-14-19

 received in 2012-09-06, accepted in 2013-03-15,  发布年份 2013

【 摘 要 】

Background

Claudins are key integral proteins of the tight junction. Although they play an essential role in controlling paracellular diffusion in epithelia, increasing evidence supports a role for these proteins in non-barrier forming activities. To elucidate a potential function for claudins outside of their traditional role in tight junctions, subcellular localization of claudin-4 was determined in normal mammary epithelial cells as well as breast and ovarian cancer cell lines and the effects of a claudin mimic peptide on cell motility were determined.

Results

Immunofluorescence revealed that claudin-4 was localized along cellular projections. Using a fluorescent peptide that mimics a conserved sequence in the second extracellular loop of a set of claudin subtypes, that includes claudin-4, exposure of this loop to the extracellular environment was confirmed in non-polarized cells. This peptide inhibited cell motility when normal mammary epithelial cells as well as breast and ovarian tumor cells were subjected to a wound healing assay. Knockdown of claudin-4 also inhibited cell motility and the mimic peptide had no effect on motility in the claudin-4 knockdown cells. This effect on motility was seen when cells were grown on collagen, but not when cells were grown on non-physiological cell adhesive or fibronectin.

Conclusion

The second extracellular loop of claudins is able to interact with the extracellular environment to promote normal and tumor cell motility when it is not associated with tight junction structures.

【 授权许可】

   
2013 Webb et al.; licensee BioMed Central Ltd.

【 图 表 】

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