An integrated “omics” approach to the characterization of maize (Zea mays L.) mutants deficient in the expression of two genes encoding cytosolic glutamine synthetase

BMC Genomics

Bertrand Hirel²  Céline Dargel-Graffin²  Thérèse Tercé-Laforgue²  Isabelle Quilleré²  Thierry Balliau⁴  Benoît Valot⁴  Michel Zivy⁴  Nicolas Agier¹  Gilles Clément²  Sandrine Imbaud³  Nardjis Amiour² 

[1] Centre National de la Recherche Sceintifique, Unité Mixte de Recherche 7238, Biologie Computationnelle et Quantitative, F-75006 Paris, France;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique (INRA), Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 INRA-Agro-ParisTech, Equipe de Recherche Labellisée, Centre National de la Recherche Scientifique 3559, RD10, F-78026 Versailles, Cedex, France;Centre de Génétique Moléculaire, Unité Propre de Recherche 2167, Centre National de la Recherche Scientifique and, Gif/Orsay DNA MicroArray Platform (GODMAP), 1, avenue de la Terrasse, F-91198, Gif sur Yvette Paris, France;Platerforme d’Analyse Protéomique de Paris Sud-Ouest, Unité Mixte de Recherche de Génétique Végétale, Ferme du Moulon, F-91190, Gif/Yvette, Paris, France

关键词: Yield;    Transcriptome;    Proteome;    Nitrogen;    Mutant;    Metabolome;    Maize;    Grain filling;    Glutamine synthetase;    Assimilation;   

Others  :  1091776
DOI  :  10.1186/1471-2164-15-1005

 received in 2014-06-19, accepted in 2014-11-04,  发布年份 2014

【 摘 要 】

Background

To identify the key elements controlling grain production in maize, it is essential to have an integrated view of the responses to alterations in the main steps of nitrogen assimilation by modification of gene expression. Two maize mutant lines (gln1.3 and gln1.4), deficient in two genes encoding cytosolic glutamine synthetase, a key enzyme involved in nitrogen assimilation, were previously characterized by a reduction of kernel size in the gln1.4 mutant and by a reduction of kernel number in the gln1.3 mutant. In this work, the differences in leaf gene transcripts, proteins and metabolite accumulation in gln1.3 and gln1.4 mutants were studied at two key stages of plant development, in order to identify putative candidate genes, proteins and metabolic pathways contributing on one hand to the control of plant development and on the other to grain production.

Results

The most interesting finding in this study is that a number of key plant processes were altered in the gln1.3 and gln1.4 mutants, including a number of major biological processes such as carbon metabolism and transport, cell wall metabolism, and several metabolic pathways and stress responsive and regulatory elements. We also found that the two mutants share common or specific characteristics across at least two or even three of the “omics” considered at the vegetative stage of plant development, or during the grain filling period.

Conclusions

This is the first comprehensive molecular and physiological characterization of two cytosolic glutamine synthetase maize mutants using a combined transcriptomic, proteomic and metabolomic approach. We find that the integration of the three “omics” procedures is not straight forward, since developmental and mutant-specific levels of regulation seem to occur from gene expression to metabolite accumulation. However, their potential use is discussed with a view to improving our understanding of nitrogen assimilation and partitioning and its impact on grain production.

【 授权许可】

   
2014 Amiour et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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