High catechin concentrations detected in Withania somnifera (ashwagandha) by high performance liquid chromatography analysis

BMC Complementary and Alternative Medicine

Siew Hua Gan¹  Siti Amrah Sulaiman²  Mohammed Moniruzzaman²  MdIbrahim Khalil²  Monzur Hossain³  Nadia Alam³ 

[1] Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia;Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia;Department of Botany, Rajshahi University, Bangladesh

关键词: free radical scavenging activity;    antioxidant;    polyphenols;    HPLC;    spectrophotometry;    Withania somnifera;   

Others  :  1232983
DOI  :  10.1186/1472-6882-11-65

 received in 2011-05-22, accepted in 2011-08-19,  发布年份 2011

【 摘 要 】

Background

Withania somnifera is an important medicinal plant traditionally used in the treatment of many diseases. The present study was carried out to characterize the phenolic acids, flavonoids and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activities in methanolic extracts of W. somnifera fruits, roots and leaves (WSFEt, WSREt and WSLEt).

Methods

WSFEt, WSREt and WSLEt was prepared by using 80% aqueous methanol and total polyphenols, flavonoids as well as DPPH radical scavenging activities were determined by spectrophotometric methods and phenolic acid profiles were determined by HPLC methods.

Results

High concentrations of both phenolics and flavonoids were detected in all parts of the plant with the former ranging between 17.80 ± 5.80 and 32.58 ± 3.16 mg/g (dry weight) and the latter ranging between 15.49 ± 1.02 and 31.58 ± 5.07 mg/g. All of the three different plant parts showed strong DPPH radical scavenging activities (59.16 ± 1.20 to 91.84 ± 0.38%). Eight polyphenols (gallic, syringic, benzoic, p-coumaric and vanillic acids as well as catechin, kaempferol and naringenin) have been identified by HPLC in parts of the plant as well. Among all the polyphenols, catechin was detected in the highest concentration (13.01 ± 8.93 to 30.61 ± 11.41 mg/g).

Conclusion

The results indicating that W. somnifera is a plant with strong therapeutic properties thus further supporting its traditional claims. All major parts of W. somnifera such as the roots, fruits and leaves provide potential benefits for human health because of its high content of polyphenols and antioxidant activities with the leaves containing the highest amounts of polyphenols specially catechin with strong antioxidant properties.

【 授权许可】

   
2011 Alam et al; licensee BioMed Central Ltd.

【 图 表 】

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