Infection routes matter in population-specific responses of the red flour beetle to the entomopathogen Bacillus thuringiensis

BMC Genomics

Joachim Kurtz¹  Erich Bornberg-Bauer¹  Hinrich Schulenburg²  Philip Rosenstiel³  Daniela Esser³  Hendrik Eggert¹  Barbara Milutinović¹  Robert Peuß¹  Sarah Behrens¹ 

[1] Institute for Evolution and Biodiversity, University of Münster, Hüfferstr. 1, 48149 Münster, Germany;Zoological Institute, Christian-Albrechts University Kiel, Am Botanischen Garten 1-9, 24118 Kiel, Germany;Institute of Clinical Molecular Biology, Christian-Albrechts University Kiel, Schittenhelmstr. 12, 24105 Kiel, Germany

关键词: Transcriptome;    RNA sequencing;    Pricking infection;    Oral infection;    Host-parasite interactions;    Bacillus thuringiensis;    Tribolium castaneum;   

Others  :  1216646
DOI  :  10.1186/1471-2164-15-445

 received in 2014-01-14, accepted in 2014-06-04,  发布年份 2014

【 摘 要 】

Background

Pathogens can infect their hosts through different routes. For studying the consequences for host resistance, we here used the entomopathogen Bacillus thuringiensis and the red flour beetle Tribolium castaneum for oral and systemic (i. e. pricking the cuticle) experimental infection. In order to characterize the molecular mechanisms underpinning the two different infection routes, the transcriptomes of beetles of two different T. castaneum populations – one recently collected population (Cro1) and a commonly used laboratory strain (SB) – were analyzed using a next generation RNA sequencing approach.

Results

The genetically more diverse population Cro1 showed a significantly larger number of differentially expressed genes. While both populations exhibited similar reactions to pricking, their expression patterns in response to oral infection differed remarkably. In particular, the Cro1 population showed a strong response of cuticular proteins and developmental genes, which might indicate an adaptive developmental flexibility that was lost in the SB population presumably as a result of inbreeding. The immune response of SB was primarily based on antimicrobial peptides, while Cro1 relied on responses mediated by phenoloxidase and reactive oxygen species, which may explain the higher resistance of this strain against oral infection.

Conclusions

Our data demonstrate that immunological and physiological processes underpinning the two different routes of infection are clearly distinct, and that host populations particularly differ in responses to oral infection. Furthermore, gene expression upon pricking infection entailed a strong signal of wounding, highlighting the importance of pricking controls in future infection studies.

【 授权许可】

   
2014 Behrens et al.; licensee BioMed Central Ltd.

【 图 表 】

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