A proteomic analysis of the regulon of the NarP two-component regulatory system response regulator in the bovine pathogen Mannheimia haemolytica A1

BMC Research Notes

Reggie Lo¹  Ichiro Inamoto¹ 

[1] Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada

关键词: LC-MS;    2D electrophoresis;    narP mutant;   

Others  :  1166923
DOI  :  10.1186/1756-0500-4-510

 received in 2011-07-11, accepted in 2011-11-24,  发布年份 2011

【 摘 要 】

Background

The response of the NarQP two-component signal transduction system regulon in response to the presence of nitrate for the bovine pathogen Mannheimia haemolytica A1 was investigated by proteomic analysis. Total proteins from a narP mutant and the parent SH1217 grown with or without NaNO₃ supplement were examined by ISO-DALT 2D electrophoresis and liquid chromatography-mass spectrometry.

Results

Seventeen proteins were differentially expressed in the parent strain SH1217 in response to the addition of NaNO₃ to the growth media. These responses were absent in the narP mutant, indicating that the altered production of these proteins is mediated by NarP_Mh. Interestingly, NarP_Mh mediated the increased production of some proteins which are not generally associated with nitrate respiration, such as the iron transporters FbpA and YfeA. The increased production of proteins such as superoxide dismutase, SodA, and GAPDH were also observed. The increased production of these iron-regulated proteins by NarP_Mh is thought to enhance the swift establishment of the nitrate respiration mechanism of M. haemolytica during pathogenesis.

Conclusion

The data suggested NarP_Mh acts as an important regulator which regulates the expression of a small set of proteins in response to nitrate availability. This may contribute to the prevalence of M. haemolytica A1 in its host during pathogenesis of BPP, through enhancing the effectiveness of nitrate respiration either directly or indirectly.

【 授权许可】

   
2011 Inamoto et al.; licensee BioMed Central Ltd.

【 图 表 】

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