Application of proteomics to identify the target molecules involved in Lonicera japonica-induced photokilling in human lung cancer CH27 cells

BMC Complementary and Alternative Medicine

Hong Z Lee²  Mann J Hour²  Yu H Lan²  Wen T Chang¹  Jung C Liao² 

[1] School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan;School of Pharmacy, China Medical University, Taichung, Taiwan

关键词: Endoplasmic reticulum chaperones;    Mitochondrial chaperones;    2D electrophoresis;    Photocytotoxicity;    Human lung squamous carcinoma CH27 cells;    Lonicera japonica;   

Others  :  1220946
DOI  :  10.1186/1472-6882-13-244

 received in 2013-07-01, accepted in 2013-09-26,  发布年份 2013

【 摘 要 】

Background

The Lonicera japonica has been used as natural and healthy drink for its anti-inflammatory effect and pleasant odor in China and Taiwan.

Methods

2D electrophoresis was used to analyze the proteins involved in photoactivated Lonicera japonica-induced CH27 cell apoptosis. The fluorescent dyes MitoTracker Red CMXRos, calcein AM and JC-1 were used to elucidate mitochondrial function. The protein expression was performed by Western blotting. Fluorescent image of endoplasmic reticulum was accomplished by using ER-Tracker Green. This study used fluorescent dye CM-H₂DCFDA to detect intracellular generation of reactive oxygen species.

Results

The identified proteins can be classified into three major groups, which include proteins involved in mitochondrial function, cytoskeleton-related proteins and proteins associated with endoplasmic reticulum (ER) stress. Photoactivated Lonicera japonica caused a significant effect on the mitochondrial function and ER stress in CH27 cells. The reactive oxygen species producing was found to be involved in photoactivated Lonicera japonica-induced CH27 cell apoptosis.

Conclusion

Mitochondria and endoplasmic reticulum are the integral targets in photoactivated Lonicera japonica-induced CH27 cell apoptosis. We also demonstrated that ethyl acetate fraction of Lonicera japonica extracts caused photocytotoxicity in a dose-dependent manner in CH27 cells. This could explain the fact that the ethyl acetate fraction of Lonicera japonica extracts may contain compounds which exhibit the photosensitizing activity in CH27 cells.

【 授权许可】

   
2013 Liao et al.; licensee BioMed Central Ltd.

【 图 表 】

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