A comparative analysis of host responses to avian influenza infection in ducks and chickens highlights a role for the interferon-induced transmembrane proteins in viral resistance

BMC Genomics

David W. Burt²  Robert G. Webster¹  Paul Digard²  J. Patrick Seiler¹  Angela F. Danner¹  Heather L. Forrest¹  Maria Weronika Gutowska²  Ian R. Paton²  Le Yu²  Nikki Smith²  Jacqueline Smith² 

[1]St. Jude Children’s Research Hospital, Virology Division, Department of Infectious Diseases, 262 Danny Thomas Place, Memphis 38105, TN, USA

[2]The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK

关键词: Evolution and gene expression profiles;    Interferon-induced transmembrane proteins;    Pathogenicity;    Chicken;    Duck;    H5N2;    H5N1;    Avian influenza;   

Others  :  1222426
DOI  :  10.1186/s12864-015-1778-8

 received in 2014-08-26, accepted in 2015-07-14,  发布年份 2015

【 摘 要 】

Background

Chickens are susceptible to infection with a limited number of Influenza A viruses and are a potential source of a human influenza pandemic. In particular, H5 and H7 haemagglutinin subtypes can evolve from low to highly pathogenic strains in gallinaceous poultry. Ducks on the other hand are a natural reservoir for these viruses and are able to withstand most avian influenza strains.

Results

Transcriptomic sequencing of lung and ileum tissue samples from birds infected with high (H5N1) and low (H5N2) pathogenic influenza viruses has allowed us to compare the early host response to these infections in both these species. Chickens (but not ducks) lack the intracellular receptor for viral ssRNA, RIG-I and the gene for an important RIG-I binding protein, RNF135. These differences in gene content partly explain the differences in host responses to low pathogenic and highly pathogenic avian influenza virus in chicken and ducks. We reveal very different patterns of expression of members of the interferon-induced transmembrane protein (IFITM) gene family in ducks and chickens. In ducks, IFITM1, 2 and 3 are strongly up regulated in response to highly pathogenic avian influenza, where little response is seen in chickens. Clustering of gene expression profiles suggests IFITM1 and 2 have an anti-viral response and IFITM3 may restrict avian influenza virus through cell membrane fusion. We also show, through molecular phylogenetic analyses, that avian IFITM1 and IFITM3 genes have been subject to both episodic and pervasive positive selection at specific codons. In particular, avian IFITM1 showed evidence of positive selection in the duck lineage at sites known to restrict influenza virus infection.

Conclusions

Taken together these results support a model where the IFITM123 protein family and RIG-I all play a crucial role in the tolerance of ducks to highly pathogenic and low pathogenic strains of avian influenza viruses when compared to the chicken.

【 授权许可】

   
2015 Smith et al.

【 图 表 】

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