Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves

BMC Research Notes

Shahnaj Parvin¹  Mamunur Rashid¹  Proma Khondkar³  AHM Khurshid Alam¹  Aziz Abdur Rahman¹  Rafikul Islam²  Ekramul Islam¹  Mahfuza khatoon¹ 

[1] Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh;Department of Pharmacy, International Islamic University of Chittagong, Chittagong, 4203, Bangladesh;Department of Pharmaceutical and Biological Sciences, UCL School of Pharmacy, London, UK

关键词: Total phenolic content;    Phytochemical constituents;    Free radical scavenging;    Antioxidants;   

Others  :  1143082
DOI  :  10.1186/1756-0500-6-121

 received in 2012-11-23, accepted in 2013-03-20,  发布年份 2013

【 摘 要 】

Background

Research on natural products has gained a wide popularity due to the potential of discovering active compounds. The antioxidant properties contained in plants have been proposed as one of the mechanisms for the observed beneficial effect. Therefore, the present study investigated the antioxidant activity and total phenolic contents of various solvent extracts of Albizia procera leaves.

Methods

Antioxidant activity of the methanol extract and its derived fractions petroleum ether (APP), carbon tetrachloride (APC), dichloromethane (APD), ethyl acetate (APE), and residual aqueous fraction (APA) of the leaves of Albizia procera was performed by in vitro chemical analyses. Total phenolic content of the APM and other five fractions were also determined. APM and its derived fractions were also subjected to preliminary phytochemical screening test for various constituents.

Results

Phytochemical screening revealed the presence of saponins, steroids, tannins, glycosides and flavonoids in the extracts. Amongst the extracts, APE showed the highest total phenolic content (449.18 ± 18.41mg of gallic acid equivalent/g of extract). In DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging test, the IC₅₀ value of APM, APP, APC, APD, APE and APA was 43.43, 63.60, 166.18, 41.15, 11.79, and 63.06 μg/mL, respectively. Therefore, among the APM and its derived fractions, APE showed the highest antioxidant activity which is comparable to that of standard ascorbic acid (AA) (IC₅₀ 10.12 μg/mL). The total antioxidant capacity was found to be varied in different fractions. The reducing activity on ferrous ion was ranked as APE > APD > APM > APA > APC.

Conclusion

The above evidences suggest that APE of A. procera leaf is a potential source of natural antioxidant and can be used to prevent diseases associated with free radicals.

【 授权许可】

   
2013 khatoon et al.; licensee BioMed Central Ltd.

【 图 表 】

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