Induction of mitochondrial dependent apoptosis and cell cycle arrest in human promyelocytic leukemia HL-60 cells by an extract from Dorstenia psilurus: a spice from Cameroon

BMC Complementary and Alternative Medicine

Ajit Kumar Saxena²  Shashi Bhushan²  Jeanne Yonkeu Ngogang³  Bathelemy Ngameni³  Suresh Kumar²  Pantaleon Ambassa¹  Santosh Kumar Guru²  Constant Anatole Pieme² 

[1] Faculty of Science, University of Yaounde I, P.O. BOX 812, Yaounde, Cameroon;Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, India;Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O. BOX 1364, Yaounde, Cameroon

关键词: Mitochondrial membrane potential;    ROS;    HL-60;    Apoptosis;    Spice;    Dorstenia psilurus;   

Others  :  1221009
DOI  :  10.1186/1472-6882-13-223

 received in 2013-05-20, accepted in 2013-08-30,  发布年份 2013

【 摘 要 】

Background

The use of edible plants is an integral part of dietary behavior in the West region of Cameroon. Dorstenia psilurus (Moraceae) is widely used as spice and as medicinal plant for the treatment of several diseases in Cameroon. The aim of this study is to investigate the cytotoxic and apoptotic potential of methanol extract of D. psilurus in human promyelocytic leukemia (HL-60) cells and prostate cancer (PC-3) cells.

Methods

Cytotoxicity of D. psilurus extract was tested in HL-60 and PC-3 cells using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay and flow cytometric methods

Results

The methanol extract of D. psilurus have significant in vitro cytotoxic activity in HL-60 cells and PC-3 cells with IC₅₀ value of 12 ±1.54 μg/ml and 18 ± 0.45 μg/ml respectively after 48 h. The mechanism of antiproliferative activity showed that after 24 h, D. psilurus extract induces apoptosis on HL-60 cells by the generation of reactive oxygen species (ROS) along with concurrent loss of mitochondrial membrane potential, modification in the DNA distribution and enhance of G2/M phase cell cycle.

Conclusion

The extract induces apoptosis of HL-60 cells associated with ROS production, loss of mitochondrial membrane potential and apoptotic DNA fragmentation.

【 授权许可】

   
2013 Pieme et al.; licensee BioMed Central Ltd.

【 图 表 】

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